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当前位置: 首页 > 文章 > Developmental changes of GHR mRNA expression level in sheep liver. 动物与饲料科学(英文版) 2009,1 (2)
Position: Home > Articles > Developmental changes of GHR mRNA expression level in sheep liver. Animal Husbandry and Feed Science 2009,1 (2)

Developmental changes of GHR mRNA expression level in sheep liver.

作  者：
Zhiguo Huang;Zhihua Xi
单  位：
College of Animal Science and Technology, Nanjing Agricultural University,Nanjing 210095;College of Animal Science and Technology, Nanjing Agricultural University,Nanjing 210095)
关键词：
days;ghr;sheep;90;30;kazak shee
摘  要：
[Objective] To detect the expression of liver growth hormone receptor gene (GHR) during the early development of Kazak sheep and Xinjiang fine-wool sheep,and thus provide information for the research about the early growth and development of sheep.[Method] With real-time quantitative PCR,the liver GHR mRNA level was separately detected in two varieties of sheep at 2,30,60,90 and 120 days old.Then the data was analyzed with SPSS software.[Result] The liver GHR gene was highly expressed in 2 day-old sheep,but the expression level fell to the lowest point at 30 days old and then rose continuously;the change trend after 30 days old was positively correlated with the cumulative growth curves of sheep (P0.05);GHR mRNA level was lower in Kazak sheep than in Xinjiang fine-wool sheep during 2-90 days old,but the difference was extremely significant (P0.01) only at 2 days old and 90 days old.[Conclusion] The male Kazak sheep and Xinjiang fine-wool sheep have similar model of developmental changes of liver GHR mRNA and the differences are smaller between these two species;GHR gene may play an important role in the regulation of sheep growth.

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