关键词：
羊羔美酒大曲;细菌;聚合酶链式反应-变性梯度凝胶电泳
摘 要：
目的:使用聚合酶链式反应-变性梯度凝胶电泳(polymerase chain reaction-denatured gradient gelelectrophoresis,PCR-DGGE)技术检测羊羔美酒大曲中的细菌,探寻酒曲中细菌菌群的多样性组成,研究羊羔美酒的风味特征。方法:对羊羔美酒大曲进行样品处理、提取总DNA、PCR扩增、DGGE条带的切胶与测序分析。结果:利用PCR-DGGE技术检测到羊羔美酒大曲中包含魏斯氏菌属(Weissella)、乳杆菌属(Lactobacillus)、片球菌(Pediococcus)、芽孢乳杆菌(Sporolactobacillus)、葡萄球菌(Staphylococcus)、高温放线菌属(Thermoactinomyces)和4个不可培养的细菌种类。结论:羊羔美酒大曲中细菌菌群多样性丰富,其中乳酸菌菌群是最大的细菌群系。
译 名：
Bacterial Diversity of Daqu for Yanggaomeijiu as Determined by PCR-Mediated DGGE
作 者：
LI Yan;DONG Zhenling;LI Jia;MOU Dehua;College of Bioscience and Bioengineering, Hebei University of Science and Technology;R&D Center for Fermentation Engineering of Hebei Province;Hebei Inatural Biological Technology Co. Ltd.;
关键词：
Yanggaomeijiu Daqu;;bacteria;;PCR-DGGE
摘 要：
Purpose: To detect and analyze the bacterial diversity of Yanggaomeijiu Daqu(traditional Chinese fermentation starter) by polymerase chain reaction-denatured gradient gelelectrophoresis(PCR-DGGE) for further understanding of the flavor characteristics of Yanggaomeijiu. Methods: Samples of Yanggaomeijiu were processed and then extracted to obtain total DNA for PCR amplification and the amplified DNA bands were excised from the gels for sequence analysis by DGGE. Results: By using PCR-DGGE, the genus of Weissella, Lactobacillus, Pediococcus, Sporolactobacillus, Staphylococcus, Thermoactinomyces and 4 unculturable bacterial strains were identified in Yanggaomeijiu Daqu. Conclusions: This work shows that the bacterial diversity is abundant in Yanggaomeijiu Daqu among which, lactic acid bacteria constituted the largest group.
