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Position: Home > Articles > Identification and Allergenicity of Allergic Proteins in Ginkgo Seeds FOOD SCIENCE 2011,32 (7) 16-20

白果过敏蛋白及其过敏原性研究

作  者:
李莹莹;吴彩娥;杨剑婷;范龚健
单  位:
安徽科技学院食品药品学院;南京林业大学森林资源与环境学院
关键词:
白果;过敏蛋白;SDS-PAGE;Western-Blotting;间接ELISA
摘  要:
对白果的主要过敏蛋白进行鉴定,并对其过敏原性进行分析。以SDS-PAGE和Western-Blotting分析鉴定白果蛋白提取液中的蛋白质组分和过敏蛋白,通过间接ELISA法检测过敏小鼠血清中sIgE的效价。白果的蛋白质条带主要有13条,其中以21kD和32kD含量最多。Western-Blotting分析结果表明,白果蛋白中存在3条过敏原蛋白,分子质量分别为21、32、36kD。当酶标二抗稀释度为1:1000,白果蛋白包被质量浓度为50μg/mL,间接ELISA测得过敏小鼠血清中sIgE的效价为1:6400。对白果蛋白进行分析鉴定,明确了白果中存在的过敏原蛋白。
译  名:
Identification and Allergenicity of Allergic Proteins in Ginkgo Seeds
作  者:
LI Ying-ying1,WU Cai-e1,YANG Jian-ting2,FAN Gong-jian1(1.College of Forest Resources and Environment,Nanjing Forestry University,Nanjing 210037,China; 2.College of Food and Drug,Anhui Science and Technology University,Fengyang 233100,China)
关键词:
ginkgo seed;allergic protein;SDS-PAGE;Western-Blotting;indirect ELISA
摘  要:
SDS-PAGE and Western-Blotting were used to identify the protein composition and allergic proteins of ginkgo seed extract,respectively.A total of 13 bands were observed in the SDS-PAGE pattern,and the fractions of 21 kD and 32 kD exhibited the highest content.Western-Blotting analysis indicated that ginkgo seeds had 3 allergic proteins,whose molecular weights were 21,32 kD and 36 kD,respectively.When the enzyme secondary antibody concentration was 1:1000 and the ginkgo protein coating concentration was 50μg/mL,the serum IgE titer of mice allergic to ginkgo seeds was detected to be 1:6400 by indirect ELISA.

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