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Biotechnology Bulletin
2009,2009
(5)
127-129+134
猪PBD-1基因乳腺特异性表达载体的构建
作 者：
黎丽荣;黄海军;高其双;董斌科;邓兵;刘艳;陶弼菲;向敏;吴建英;蒋思文
单 位：
武汉市畜牧兽医科学研究所;华中农业大学
关键词：
猪β防御素1;山羊乳球蛋白;乳腺特异性表达载体
摘 要：
为构建猪PBD-1基因乳腺特异性表达载体,采用PCR方法从质粒pcDNA3.1-BLG-HNP-1中扩增出山羊乳球蛋白(BLG)基因,插入到真核表达载体pIRES2-EGFP-PBD-1构建成乳腺特异性表达载体pIRES2-EGFP-BLG-PBD-1。经PCR鉴定、限制性内切酶酶切分析和克隆片段序列测定、比较,鉴定。结果成功构建了乳腺特异性表达载体pIRES2-EGFP-BLG-PBD-1。乳腺特异性表达载体pIRES2-EGFP-BLG-PBD-1的成功构建,为进一步研究PBD-1蛋白的抗菌活性、抗菌机理及将进一步该载体应用于动物乳腺生物反应器的研究奠定基础。
译 名：
Construction of the Mammary Gland Special Expression Vector for the Porcine Beta-defensin 1
作 者：
Li Lirong1,2 Huang Haijun1 Gao Qishuang3 Dong Binke1 Deng Bing1Liu Yan1 Tao Bifei3 Xiang Min3 Wu Jianying3 Jiang Siwen1(1Huazhong Agricultural University Key Laboratory of Swine Breeding and Genetics,Ministry of Agriculture& Key Laboratory of Agricultural Animal Genetics,Breeding and Reproduction,Wuhan 430070;2Xianning Vocational TechnicalCollege,Xianning 437100;3Wuhan Institute of Animal and Veterinary Science,Wuhan 430065)
关键词：
PBD-1 BLG Mammary gland special expression vector
摘 要：
It was to construct a mammary gland special expression vector for the porcine beta-defensin 1.BLG gene was amplified from the vector of pcDNA3.1-BLG-HNP-1 and inserted into a eukaryotic expression plasmid pIRES2-EGFP-PBD-1.The recombinant colonies were identified by the methods of restriction enzyme digestion,PCR and sequencing.Results showed that the mammary gland special expression vector pIRES2-EGFP-BLG-PBD-1 was successfully constructed.The establishment of mammary gland special expression vector pIRES2-EGFP-BLG-PBD-1 would lays a foundation in research on antimicrobia1 activities and its mechanism of the defensins,and also would benefit further research on the establishment of animal mammary bioreactor.
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