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Position: Home > Articles > Cloning and Site-directed Modification of Sorghum bicolor EPSPS Gene and Its Functional Validation in Maize Journal of Maize Sciences 2013 (6) 5-9

高粱EPSPS基因的克隆、修饰及在玉米中的功能验证

作  者:
赵海铭;宋伟彬;赖锦盛
单  位:
中国农业大学/农业生物技术国家重点实验室/国家玉米改良中心
关键词:
玉米;转基因;EPSPS;抗草甘膦
摘  要:
针对草甘膦结合位点,对高粱5-烯醇式丙酮酰莽草酸-3-磷酸合酶(EPSPS)基因进行4种定点修饰,将修饰后的基因分别导入到玉米中。通过对转化体的抗性鉴定,确定将高粱EPSPS基因106位的脯氨酸变为丝氨酸(P106S)能够赋予转基因玉米草甘膦抗性。在喷施4倍的草甘膦时抗性事件CL38-1不产生药害。Southern杂交结果表明,目标基因在该转化事件中稳定遗传,转化其余3种EPSPS基因的植株对草甘膦没有足够的抗性。
译  名:
Cloning and Site-directed Modification of Sorghum bicolor EPSPS Gene and Its Functional Validation in Maize
作  者:
ZHAO Hai-ming;SONG Wei-bin;LAI Jin-sheng;China Agricultural University, State Key Laboratory of Agrobiotechnology/National Maize Improvement Center;
关键词:
Maize;;Transgene;;EPSPS;;Glyphosate-resistant
摘  要:
According to the potential binding site of glyphosate, Sorghum 5-enolpyruvylshikimate-3-phosphate synthase(SbEPSPS) and conducted four kinds of site-directed modification were cloned. These modified SbEPSPS were introduced in maize using transgenic approach. We found that the transgenic maize with one amino acid substitution: proline(P106) to serine(S) of EPSPS gained glyphosate resistance. One of transgenic events CL38-1 showed glyphosate resistant after sprayed with 4-fold glyphosate. Southern blot analysis of transformant showed that the transgene inherited stably. Plants transformed with other three types of modified SbEPSPS genes did not show enough glyphosate resistance.

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