Position: Home > Articles > Effect of γ-L-glutamoyl-S-allyl-L-cysteine on Mitochondrial Function of Hepatic Stellate Cells
Journal of Anhui Agricultural Sciences
2015
(35)
220-222+255
γ-L-谷氨酰-S-烯丙基-L-半胱氨酸对肝星状细胞线粒体功能的影响
作 者：
张笔觅;孙瑞波;吕智超;杨明静;李万良
单 位：
吉林省农业科学院;吉林省经济管理干部学院
关键词：
γ-L-谷氨酰-S-烯丙基-L-半胱氨酸;肝星状细胞;线粒体膜电位;MTT
摘 要：
[目的]通过研究γ-L-谷氨酰-S-烯丙基-L-半胱氨酸(GSAC)对大鼠肝星状细胞(HSC-T6)线粒体功能的影响,探讨GSAC对肝纤维化的抑制作用。[方法]利用微板法测定GSAC对培养24 h细胞线粒体代谢甲基噻唑基四唑(MTT)的影响,以罗丹明123作为染料,通过流式细胞仪检测线粒体膜电位。[结果]GSAC具有抑制HSC-T6线粒体代谢MTT的作用,IC50值为0.835 mg/ml;GSAC引起线粒体膜电位崩溃,所有GSAC处理组(0.2、0.4与0.8 mg/ml)罗丹明123荧光强度极显著高于对照组。[结论]GSAC可破坏HSC-T6细胞线粒体功能,提示其对肝纤维化具有抑制作用。
译 名：
Effect of γ-L-glutamoyl-S-allyl-L-cysteine on Mitochondrial Function of Hepatic Stellate Cells
作 者：
ZHANG Bi-mi;SUN Rui-bo;LV Zhi-chao;LI Wan-liang;Jilin Province Economic Management Cadre College;Jilin Academy of Agricultural Sciences;
关键词：
γ-L-glutamoyl-S-allyl-L-cysteine;;HSC-T6 cells;;Mitochondrial membrane potential;;MTT
摘 要：
[Objective] The research aimed to explore inhibition effect of γ-L-glutamoyl-S-allyl-L-cysteine( GSAC) on liver fibrosis by studying the influence of GSAC on the mitochondrial function of rat hepatic stellate cells( HSC-T6). [Method]After 24 h culture in different concentrations of GSAC,methyl thiazolyl tetrazolium( MTT) metabolism was tested,with the median inhibitory concentration( IC50) value obtained in a microplate assay,and mitochondrial membrane potential( MMP) was tested in a flow cytometer assay with Rhodamine 123 as indicator. [Result]GSAC inhibited MTT metabolism,with IC50= 0. 835 mg / ml,and GSAC inhibited MMP,the fluorescence intensity in GSAC treated groups( 0. 2,0. 4 and 0. 8 mg / ml) significantly increased compared to the control. [Conclusion] GSAC damages mitochondrial function of HSC-T6 cells,which suggests that GSAC has an inhibitory effect on liver fibrosis.
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