Position: Home > Articles > Effect of Propyl p-Hydroxybenzoate on SOD Gene Expression of in Drosophila melanogaster
FOOD SCIENCE
2011,32
(19)
170-173
对羟基苯甲酸丙酯对果蝇超氧化物歧化酶(SOD)基因表达的影响
作 者：
蒋芳平;应琼琼;顾蔚
单 位：
陕西师范大学生命科学学院
关键词：
对羟基苯甲酸丙酯(PP);果蝇;超氧化物歧化酶(SOD);基因表达
摘 要：
探讨对羟基苯甲酸丙酯(PP)对果蝇超氧化物歧化酶(SOD)基因表达的影响,用含不同质量浓度(0.00、0.20、0.50、1.00、2.00g/L)的PP培养基饲喂果蝇10d后,提取不同PP组果蝇总RNA,采用逆转录PCR方法在mRNA表达水平上研究SOD基因。结果表明:PP抑制了果蝇体内抗氧化酶Cu,Zn-SOD、Mn-SOD基因的表达,与对照组相比具有显著性差异(P<0.05);随着PP质量浓度的升高,雌果蝇体内Cu,Zn-SOD基因与雄果蝇体内Mn-SOD基因的表达量随之降低,而Mn-SOD基因在雌果蝇体内总体表达量提高。故PP的毒性作用可能与抑制抗氧化酶基因的表达有关,而这种作用因果蝇性别而异。
译 名：
Effect of Propyl p-Hydroxybenzoate on SOD Gene Expression of in Drosophila melanogaster
作 者：
JIANG Fang-ping,YING Qiong-qiong,GU Wei(College of Life Science,Shaanxi Normal University,Xi,an 710062,China)
关键词：
propyl p-hydroxybenzoate(PP);Grosophila melanogaster;superoxide dismutase(SOD);gene expression
摘 要：
In order to explore the effect of propyl p-hydroxybenzoate(PP) on superoxide dismutase(SOD) gene expression in Drosophila melanogaster,PP was used to feed Drosophila melanogaster at the concentrations of 0,0.20,0.50,1.00 g/L and 2.00 g/L for 10 consecutive days,respectively.Total RNA was extracted from Drosophila melanogaster and RT-PCR was used to investigate the impact of PP on mRNA expression level of SOD gene in Drosophila melanogaster.The results suggested that PP could significantly suppress Cu,Zn-SOD and Mn-SOD in comparison with the control group(P< 0.05).As the PP concentration increased,the expression level of Cu,Zn-SOD gene in female flies and Mn-SOD gene in males revealed a decrease;however,the expression level of Mn-SOD gene in female flies exhibited an obvious improvement.These results indicated that the toxicological effect of PP may be related to the inhibition of SOD gene expression in Drosophila melanogaster,which shows differences between males and females.
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