Position: Home > Articles > Comparison of Protein Extraction Methods for Angelica dahurica Seeds
Journal of Anhui Agricultural Sciences
2017,45
(6)
118-120+123
白芷种子蛋白质提取方法的比较
作 者：
吴萍;郭俊霞;李青苗;夏燕莉;张美;肖特;方清茂
单 位：
四川省中医药科学院
关键词：
白芷种子;蛋白质提取;聚丙烯酰氨凝胶电泳
摘 要：
[目的]建立适用于白芷种子的SDS-PAGE蛋白质样品提取方法。[方法]比较5种提取方法所获得白芷种子总蛋白质产量以及SDS-PAGE电泳的谱带数目、强度和电泳分辨率等方面的差异。[结果]方法 a所提蛋白质含量高,杂质少,电泳谱带清晰;方法 b所提蛋白质溶液电泳谱带清晰,分辨率较高,但提取含量较低;方法 c和d所提蛋白质杂质较多,纯度不高,分辨率较低;方法 e所提蛋白质纯度较高,但蛋白条带缺失较多。[结论]方法 a提取白芷种子蛋白质的效果最好,所提蛋白效率较高,杂质少,电泳谱带清晰,分辨率高,谱带数多,优于其他提取法。
译 名：
Comparison of Protein Extraction Methods for Angelica dahurica Seeds
作 者：
WU Ping;GUO Jun-xia;LI Qing-miao;Sichuan Academy of Traditional Chinese Medicine Sciences;
关键词：
Angelica dahurica seed;;Protein extraction;;Polyacrylamide gel electrophoresis
摘 要：
[Objective] To establish a suitable protein extraction protocol for total proteins extraction from Angelica dahurica seeds. [Method]We compared the total protein yield and SDS-PAGE electrophoretic band number and strength,lectrophoresis resolution by five different methods. [Result] Method a had higher protein content,less impurity and clearer electrophoresis band. Method b had clearer electrophoresis band,higher resolution,but lower protein content. Methods c and d had more impurity,lower purity and resolution. Method e had higher protein purity,but more lack of protein bands. [Conclusion]Method a has the best extraction effects of protein from A. dahurica seeds,which has higher protein extraction efficiency,less impurity,clearer band,higher resolution,more band number. Thus,method a is superior to other four methods.
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