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当前位置: 首页 > 文章 > 食品中桃仁和杏仁过敏原成分的检测 食品科学 2010,31 (18) 220-223
Position: Home > Articles > Allergen Detection of Almond and Peach Seeds in Food FOOD SCIENCE 2010,31 (18) 220-223

食品中桃仁和杏仁过敏原成分的检测

作  者：
张霞;张海英;高旗利;刘培;张宏伟;郑文杰
单  位：
天津出入境检验检疫局
关键词：
杏仁;Prudu1基因;荧光定量PCR
摘  要：
目的:建立检测食品中桃仁、杏仁过敏原成分的荧光PCR方法,比较国外3种ELISA试剂盒效果。方法:针对杏仁Prudu1基因设计引物及探针,建立荧光PCR方法。利用杏仁过敏原参考物质对3个品牌的ELISA试剂盒的回收率进行比较。结果:建立的荧光PCR方法,具有很好的特异性;灵敏度为10mg/kg。结论:桃仁及杏仁过敏原成分荧光PCR检测方法特异性好、灵敏度高,对食品中过敏原的检测有重要的实际意义。
译  名：
Allergen Detection of Almond and Peach Seeds in Food
作  者：
ZHANG Xia,ZHANG Hai-ying,GAO Qi-li,LIU Pei,ZHONG Hong-wei,ZHENG Wen-jie(Tianjin Entry-Exit Inspection and Quarantine Bureau,Tianjin 300461,China)
关键词：
almond;allergenic Pru du1 gene;fluorescence quantitative polymerase chain reaction
摘  要：
Objectives:To establish a real-time PCR method for the detection of allergenic almond and peach seeds in food and compare with quantitative test kits for allergenic almond.Methods:On the basis of sequence analysis of the Pru du1 region,specific primers and probes were designed to develop a real-time PCR method using TaqMan probe for the identification of allergenic almond or peach seed residues in food.In order to compare with the test kits,recovery rates and detection limit of established real-time PCR were determined using the reference material of allergenic almond.Results:The established real-time PCR method was highly specific for the detection of allergenic almond.Its sensitivity was less than 10 mg/kg.Conclusion:The real-time PCR method is highly sensitive and specific,thereby providing an applicable method for the detection of allergenic almond and peach seeds in food.

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