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Position: Home > Articles > A Tentative Research of Alleviating Oxidative Damage of Exogenous Nitric Oxide to Dunaliella salina under Salinity Stress Journal of Henan Agricultural Sciences 2009,38 (5) 47-52

外源一氧化氮缓解盐胁迫下杜氏盐藻氧化损伤的初步研究

作  者:
卢雪景;刘红涛;柴玉荣;朱大恒;罗清菊;吕鹏举;薛乐勋
单  位:
郑州大学生物工程系细胞生物学研究室
关键词:
盐胁迫;硝普钠;杜氏盐藻;氧化损伤
摘  要:
研究外源一氧化氮(NO)供体硝普钠(sodium nitroprusside,SNP)缓解3.0 mol/L NaCl胁迫对杜氏盐藻的氧化损伤作用并探讨其初步机理。结果表明:与单独盐胁迫相比,添加低浓度的SNP(0.5μmol/L和1μmol/L)能促进杜氏盐藻的生长,显著提高其叶绿素含量,并增强了超氧化物歧化酶(SOD)的活性(P<0.05)。其中,1μmol/L SNP处理24 h后SOD活性提高了1.6倍。此外,半定量RT-PCR结果显示,低浓度的SNP(0.5μmol/L和1μmol/L)对盐胁迫下杜氏盐藻Fe-SOD基因的转录具有促进作用,24 h时作用显著(P<0.05),表达量分别提高了21.18%和27.41%,而高浓度的SNP(50μmol/L)抑制Fe-SOD基因的表达,加剧了盐胁迫带来的氧化伤害。
译  名:
A Tentative Research of Alleviating Oxidative Damage of Exogenous Nitric Oxide to Dunaliella salina under Salinity Stress
作  者:
LU Xue-jing,LIU Hong-tao,CHAI Yu-rong,ZHU Da-heng,LUO Qing-ju,LU Peng-ju,XUE Le-xun*(Department of Bioengineering of Zhengzhou University,Zhengzhou 450001,China)
关键词:
Salinity stress;Sodium nitroprusside;Dunaliella salina;Oxidative damage
摘  要:
A tentative research of alleviating the oxidative damage of sodium nitroprusside(SNP),a nitric oxide(NO) donor,to Dunaliella salina under salinity stress of 3.0 mol/L NaCl was explored in this study.The results showed that the addition of low concentrations of SNP(0.5 μmol/L和1 μmol/L) not only promoted the proliferation of Dunaliella salina and increased the content of chlorophyll significantly,but also enhanced the activity of superoxide dismutase(SOD)(P<0.05),gaining 1.6 times higher after the application of 1μmol/L SNP at 24h,compared to sole salinity stress.Furthermore,the results of Semi-quantitative RT-PCR indicated that transcription of the Fe-SOD gene was enhanced significantly by exogenous low concentration SNP(0.5μmol/L和1μmol/L) at 24h(P<0.05),with 21.18% and 27.41%,respectively.The application of high concentration SNP(50μmol/L) inhibited the expression of the Fe-SOD gene,aggravating the oxidative damage on Dunaliella salina under salinity stress.

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