当前位置: 首页 > 文章 > 芸豆蛋白质的提取及超滤分离研究 食品科学 2008,29 (8) 386-390
Position: Home > Articles > Study on Extraction and Separation of Protein from Phaseolus vulgaris by Ultra Filtration Technology FOOD SCIENCE 2008,29 (8) 386-390

芸豆蛋白质的提取及超滤分离研究

作  者:
周大寨;朱玉昌;周毅锋;黄伟
单  位:
湖北民族学院;湖北民族学院生物科学与技术学院
关键词:
芸豆;蛋白质;提取;膜分离
摘  要:
在单因素试验基础上用正交试验对芸豆蛋白的提取工艺条件进行筛选,得出最优提取条件为:在40℃下,用20倍于芸豆粉的pH10.00.067mol/L磷酸缓冲液,提取60min。同时探讨了芸豆蛋白超滤分离纯化工艺,结果表明,在压力差0.3MPa、泵功率20%、料液浓度4%、料液pH10.0条件下,经过四次超滤后,蛋白质纯度稳定在85%左右。
译  名:
Study on Extraction and Separation of Protein from Phaseolus vulgaris by Ultra Filtration Technology
作  者:
ZHOU Da-zhai1,ZHU Yu-chang1,ZHOU Yi-feng2,HUANG Wei2(1.Key Laboratory of Biologic Resources Protection and Utilization of Hubei Province,Hubei University for Nationalities,Enshi 445000,China;2.School of Biological Science and Technology,Hubei University for Nationalities,Enshi 445000,China)
关键词:
Phaseolus vulgaris;protein;extraction;separation by ultra filtration technology
摘  要:
Based on single factor tests,extraction conditions of protein from Phaseolus vulgaris were discussed and the optimum extraction conditions were determined by orthogonal test. The results of orthogonal test showed that the optimum extraction conditions are as follows:temperature 40 ℃,extraction time 60 minutes and proportion of materials to 0.067 mol/L PBS at pH 10.0 1:20. The application of ultra filtration technology in purification of protein was also discussed. On the conditions of pressure 0.3 MPa,pump power 20% and solution at pH 10.0,concentration 4% after four times ultra filtration,the protein purity is up to 85%.

相似文章

计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊