中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 肿瘤转移抑制因子(Kisspeptin)对牛卵巢颗粒细胞凋亡的影响 中国畜牧杂志 2017,53 (9) 57-61
Position: Home > Articles > Effect of Kisspeptin on the Apoptosis of Bovine Granulosa Cells Chinese Journal of Animal Science 2017,53 (9) 57-61

肿瘤转移抑制因子(Kisspeptin)对牛卵巢颗粒细胞凋亡的影响

作  者：
王军;刘红羽;徐高青;吕文发
关键词：
Kisspeptin;牛;颗粒细胞;凋亡
摘  要：
肿瘤转移抑制因子(Kisspeptin)在颗粒细胞上有表达,但其对颗粒细胞凋亡的作用尚不清楚,本试验旨在利用流式细胞术和荧光定量PCR的技术研究Kisspeptin对牛卵巢颗粒细胞凋亡的影响。向DMEM-F12培养液中分别添加0、100 nmol/L的Kp-10培养牛颗粒细胞,24 h后收集细胞,用流式细胞技术检测细胞凋亡和细胞周期,用荧光定量PCR技术检测Caspase-3、Bcl-2、Fas和Fasl的m RNA表达。结果表明:Kisspeptin可显著促进牛颗粒细胞凋亡,且伴随着Caspase-3、Fas和Fasl m RNA表达上调及Bcl-2 m RNA表达下调(P<0.01);流式细胞分析表明,Kisspeptin可使牛颗粒细胞在G1期增加、S期下降(P<0.05)。研究结果说明,Kisspeptin可促进牛颗粒细胞凋亡,这一作用可能通过上调促凋亡基因Caspase-3、Fas、Fasl和下调抗凋亡基因Bcl-2的m RNA表达实现。
译  名：
Effect of Kisspeptin on the Apoptosis of Bovine Granulosa Cells
作  者：
WANG Jun;LIU Hong-yu;XU Gao-qing;LV Wen-fa;Jilin Province Engineering Laboratory for Ruminant Reproductive Biotechnology and Healthy Production, College of Animal Science and Technology, Jilin Agricultural University;
关键词：
Kisspeptin;;Bovine;;Granulosa cells;;Apoptosis
摘  要：
Previous studies showed that Kisspeptin expresses in granulosa cells, however, its effect on apoptosis of bovine granulosa cells is still unknown. In the present study, flow cytometry analysis and q Real Time quantitative-PCR were used to study effect of Kisspeptin on apoptosis of bovine granulosa cells. Bovine granulosa cells were cultured with DMEM-F12 solution added with 0 or 100 nmol/L of Kp-10 for 24 h, then flow cytometry was used to study apoptosis and cell cycleof the bovine granulosa cells, and Real Time quantitative-PCR was used to study m RNA expression of Caspase-3, Bcl-2, Fas and Fasl. The results showed that Kisspeptin could promote apoptosis of bovine granulosa cells, accompanied with the decreased m RNA levels of Bcl-2 and the increased m RNA levels of Caspase-3, Fas and Fasl(P<0.01). Additionally, results of flow cytometry analysis showed that Kisspeptin could significantly increase accumulation of cells in the G1 phase, decrease accumulation of cells in the S phase, and promote apoptosis of bovine granulosa cells(P< 0.05). Thus, our findings demonstrated for the first time that Kisspeptin promoted apoptosis of bovine ovarian granulosa cells, which may be achieved through regulation of Caspase-3, Fas, Fasl and Bcl-2 m RNA expression.

相似文章

导出引用
计量
文章访问数: 13
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46