中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 花椰菜与黑芥体细胞杂种的生物学特性 华北农学报 2008,23 (4) 100-104
Position: Home > Articles > Biological Characters of Somatic Hybrids between Brassica oleracea L.var.botrytis and Brassica nigra Acta Agriculturae Boreali-Sinica 2008,23 (4) 100-104

花椰菜与黑芥体细胞杂种的生物学特性

作  者：
郝薇薇;赵泓;盛小光;陈斌;刘凡
单  位：
首都师范大学;北京市农林科学院蔬菜研究中心
关键词：
花椰菜;黑芥;非对称体细胞杂种;生物学特性
摘  要：
对经UV处理,PEG融合获得的79株花椰菜和黑芥的体细胞杂种进行了全生育期性状调查分析,包括细胞DNA含量测定、染色体计数、植株形态学观察、育性调查等。结果表明:杂种形态变异广泛,多数呈中间类型,少数呈偏亲性状;杂种细胞DNA含量不一致,呈现大于、等于、小于双亲细胞DNA含量之和的类型;DNA含量大于双亲之和的杂种与DNA含量等于或小于双亲之和的杂种相比,有较高比例的材料叶型出现畸形和(或)植株生长势弱,育性也更差。显示出杂种植株的生长势、育性与细胞的DNA含量具有一定的相关性。通过染色体计数,发现所检测杂种为混倍体,同一植株的细胞有多种染色体数目构成。在79个杂种中,筛选出13个目标材料,生长势强,有较好或一定程度的育性,表明采用体细胞杂交技术手段来改良甘蓝类蔬菜的遗传多样性具有可行性。
译  名：
Biological Characters of Somatic Hybrids between Brassica oleracea L.var.botrytis and Brassica nigra
作  者：
HAO Wei-wei1,2,ZHAO Hong2,SHENG Xiao-guang2,CHEN Bin2,LIU Fan2(1.Captical Normal University,Beijing 100037,China;2.Beijing Vegetable Research Center,Beijing 100097,China)
关键词：
Brassica oleracea L.var.botrytis;Brassica nigra;Asymmetric somatic hybrids;Biological characters
摘  要：
Biological characters of 79 asymmetric somatic hybrids obtained through protoplast fusion between Brassica oleracea L.var.botrytis(2n=18,CC)and Brassica nigra(2n=16,BB)were studied.The results indicated that the morphology of hybrids were heterogeneous.Most of the population was intermediate to that of the parents,compared with minority of them were similar to parents.Nuclear DNA content analysis revealed that hybrid plants had different nuclear DNA content:higher,lower than the parental sum or equal to it.Contrast to the lower and equal hybrids,bigger amount of hybrids with higher DNA content had abnormal leaf,bad growth vigour and poor fertility.It showed that growth vigour and fertility had relations with the nuclear DNA content.By chromosome counting,the detected hybrids were mixoploid.Among 79 hybrids,13 aimed hybrids were picked out,which were vigorous and fertile.Broaden the genetic variability of cabbage though asymmetric somatic hybridization was indicated feasible.

相似文章

导出引用
计量
文章访问数: 6
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46