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当前位置: 首页 > 文章 > 饲料中的转基因成分在蛋鸡体内代谢残留的研究 福建畜牧兽医 2007,29 (5) 1-4
Position: Home > Articles > PCR Analysis on the Metabolic Remains of Transgenic Component Derived from Feed in the Bodies of Laying Hens Fujian Journal of Animal Husbandry and Veterinary Medicine 2007,29 (5) 1-4

饲料中的转基因成分在蛋鸡体内代谢残留的研究

作  者：
陈文炳;王长康;江树勋;杨婕;李寿崧;郭立新
单  位：
福建农林大学动物科学学院;福建出入境检验检疫局
关键词：
转基因饲料;转基因成分;蛋鸡;代谢残留
摘  要：
用含20%抗草甘膦转基因豆粕的饲料喂养鸡蛋,通过检测其内脏、肌肉、血液、粪便及肠道微生物的豆粕转基因成分,探讨饲料中转基因成分在鸡蛋体内的代谢残留状况。以定性PCR检测含20%转基因豆粕的饲料及蛋鸡的8种内脏样品(肠、胰腺、食道、肝、腺胃、肾、心脏、肌胃)、肌肉、血液、蛋品、粪便、肠道微生物的转基因豆粕的工具基因CaMV35S启动子与NOS终止子以及目的基因CP4-EPSPS等外源基因成分。PCR检测结果表明:除了含20%转基因豆粕的饲料外,蛋鸡的8种内脏器官、肌肉、血液、蛋品、粪便及肠道微生物中均未检出转基因豆粕的CaMV35S、NOS、CP4-EPSPS等外源基因成分。检测结果表明:长期喂养含20%转基因豆粕饲料的蛋鸡的8种内脏器官、肌肉、血液、蛋品、粪便及肠道微生物中均未发现有转基因成分残留,说明饲料中的转基因成分,通过肠胃消化降解后吸收,不会在鸡的组织器官内残留。
译  名：
PCR Analysis on the Metabolic Remains of Transgenic Component Derived from Feed in the Bodies of Laying Hens
作  者：
Chen Wenbing1 Wang Changkang2 Shao Biying1 Jiang Shuxun1 Yang Jie1 Li Shousong1 Guo Lixin1(1 Fujian Entry-Exit Inspection and Quarantine Bureau,Fuzhou, Fujian, China 350001);2 College of Animal Science,Fujian Agriculture and Forestry University,Fuzhou,Fujian,China 350002)
关键词：
genetically modified feed transgenic component laying hens metabolic remains
摘  要：
The present study were to probe into the metabolic remains of transgenic component derived from feed in the 8 inner organs including intestines,pancreas,esophagus,liver,preventriculus,kidney,heart,gizzard as well as muscle,blood,egg,dejecta,intestinal bacteria of laying hens fed with feed containing 20% genetically modified soybean cake.The laying hens fed by feed containing 20% genetically modified soybean cake were used as test materials.The transgenic component including CaMV35S、NOS、CP4-EPSPS derived from feed were analyzed by the qualitification PCR. These transgenic components were found only in feed containing 20% genetically modified soybean cake,but were not found in the 8 inner organs,muscle,blood,egg,dejecta,intestinal bacteria of laying hens tested.The results indicated that the metabolic remains of transgenic components from genetically modified soybean cake in feed were not found in laying hens products because of being digested fully.

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