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Position: Home > Articles > DNA Extraction and Establishment of RAPD Analytic Conditions of Rosa multiflora Northern Horticulture 2007,2007 (6) 220-222

野蔷薇的DNA提取和RAPD反应体系的建立

作  者:
管晓庆;王奎玲;刘庆华;刘庆超;郭宝太
单  位:
山东莱阳农学院环境艺术学院;山东莱阳农学院生命科学院
关键词:
野蔷薇;DNA提取;RAPD
摘  要:
以野蔷薇(Rosa multiflora)的冬末初春芽和夏初叶片为材料,研究了DNA的提取方法,并对影响随机扩增多态DNA(RAPD)的反应因素进行了优化。建立了适合野蔷薇RAPD的反应体系和反应程序,在20μL反应体系中,25ng模板DNA,2.0mmol/LMgCl2,0.2mmol/LdNTPs,1UTaqDNA聚合酶,0.1μmol/L引物。扩增程序为:94℃预变性4min,然后94℃变性45s,37℃退火1min,72℃延伸1min,37个循环,最后72℃延伸5min,4℃保存。
译  名:
DNA Extraction and Establishment of RAPD Analytic Conditions of Rosa multiflora
作  者:
GUAN Xiao-qing1,WANG Kui-ling1,LIU Qing-hua1,LIU Qing-chao1,GUO Bao-tai2(1.Landscape Architecture and Art College,Laiyang Agricultural University,Qingdao 266109;2.Life Sciences College,Laiyang Agricultural University,Qingdao 266109)
关键词:
Rosa multiflora;DNA extraction;Random amplyfied polymorphic DNA
摘  要:
DNA of Rosa multiflora was extracted from the late-winter buds and the early-summer leaves.The optimized reaction system and programe of RAPD for Rosa multiflora were as follows.The reactions were performed in a volume of 20 L,which containing 25ngDNA,2.0mmol/LMgCl2,0.2mmol/LdNTPs,1UTaq DNA polymerase,0.1 mol/L primer.The malcycling was programmed by 1 cycle for 5min at 94℃,followed by 37 cycles for 45sec at 94℃,1min at 37℃,and 1min at 72℃ and finally,by 1 cycle for 5min at 72℃,storing at 4℃.

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