Yan Luo;Ji-Ping Kang;Jin Cheng;Likou Zou;Bei Li;Xiaohu Dai;Wang ChengXu;Guang-Min Y

Sichuan Institute of Musk Deer Breeding, Dujiangyan 611830, Chin;Qingdao Contient Pharmaceutical Group Boitech Biopharmaceutical Co

purulent;musk deer;pneumonia;disease virus;the virus;isolate

[Objective] To understand the physicochemical properties of musk deer pneumonia and purulent disease viruses. [Method] The pneumonia and purulent disease viruses were isolated from the abnormal and purulent lung tissues of musk deer. Then the isolated viruses were inoculated into the Vero cells. After culturing, the virus solution was collected and used to determine TCID50 and genome types. The sensitivity to fat solvent, resistance to hydrochloric acid and trypsin as well as tolerance to heat of the musk deer pneumonia and purulent disease viruses were detected, respectively. [Result] The obvious cytopathic effects (CPE) were found in Vero cells infected by the isolated viruses. The virus was 2-1.43 TCID50/ml and its genome was RNA. The virus was not sensitive to chloroform, 1% trypsin and heats, and it had a certain tolerance to 0.1 mol/L hydrochloric acid. [Conclusion] The study on the physicochemical properties of musk deer pneumonia and purulent disease viruses lays a foundation for prevention and control of the musk deer pneumonia and purulent diseases.