Position: Home > Articles > Study on Classification Consistency Based on Morphology and rDNA ITS Sequences of Pueraria Species
Hubei Agricultural Sciences
2016,55
(4)
939-942
葛属植物基于形态学和rDNAITS分类的一致性比较研究
作 者：
蒋向辉;刘良科;佘朝文
单 位：
湖南省怀化学院;怀化学院生物工程系;怀化学院生命科学系
关键词：
葛属(Pueraria DC);形态学;rDNA;ITS序列;分类一致性
摘 要：
试验以来自不同地区的葛属(Pueraria DC)植物11个种质作为材料,基于其叶色、茎粗、茎节长、茎色、茎粗、茎被毛等9个形态性状进行形态学分类,并对11个种质的r DNA ITS序列进行克隆测序和序列比较分析,采用MEGA 6.0软件构建ITS序列系统进化树。结果表明,基于ITS序列和基于形态特征的分类结果虽有一定的相似性,但仍存在较大的差异。不同分类方法导致的结果差异可能与形态特征受其生长条件、性状的选取等多方面因素的影响有关,而基于r DNA ITS序列的分类结果更为可靠。
译 名：
Study on Classification Consistency Based on Morphology and rDNA ITS Sequences of Pueraria Species
作 者：
JIANG Xiang-hui;LIU Liang-ke;SHE Chao-wen;College of Chemistry and Materials Engineering,Kaili University;Department of Life Science,Huaihua University;
关键词：
Pueraria DC;;morphology;;rDNA;;ITS sequences;;classification consistency
摘 要：
11 germplasms of Pueraria DC from different regions were classifed by morphological classification method based on9 morphological characters including leaf color, stem thickness, internodes length, stem color, stem diameter, stem hair;meanwhile, cluster analysis was conducted by MEGA 6.0 software based on r DNA ITS sequences of the 11 Pueraria species.It was found that the result of cluster analysis and morphological classification method had some similarities while the differences were obvious. The research suggested that the differences between different classification methods might be related to the morphological traits which could be affected by growth conditions, as well as the character selected; thus the classification result based on r DNA ITS was more reliable.
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