中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 环形泰勒焦虫裂殖体细胞培养和裂殖体胶冻细胞苗免疫效力的研究 中国农业科学 1982,15 (4) 88-95+99
Position: Home > Articles > STUDIES ON THE CELL CULTURES OF THEILERIA ANNULATA SCHIZONTS AND THE IMMUNITY INDUCED BY THE GELATINPROTECTED, SCHIZONT-PARASITIZED CELL VACCINE Scientia Agricultura Sinica 1982,15 (4) 88-95+99

环形泰勒焦虫裂殖体细胞培养和裂殖体胶冻细胞苗免疫效力的研究

作  者：
吕文顺;李观娣;吕文祥;白启;田广孚;赵开元;刘正语;马玉琳;张中行
单  位：
中国农业科学院兰州兽医研究所
摘  要：
环形泰勒焦虫(Theileria annulata)病是我国北方地区对牛危害较大的一种血液寄生虫病,其传播者是缺缘璃眼蜱(Hyalomma detritum),往往引起牛只的严重发病和死亡。国内外对本病曾做过不少研究,但始终未找到一种特效的治疗药物和可靠的预防方法。 在泰勒焦虫病的免疫研究中,(1955)用配子体(gametocyte)制苗未能成功。等(1966)认为保护性抗体的产生与裂殖体(schizont)有关。兰州兽医研究所等(1972)通过试验肯定了裂殖体具有良好的免疫原性。目前,细胞培养的方法为裂殖体疫苗的制造提供了新手段。
译  名：
STUDIES ON THE CELL CULTURES OF THEILERIA ANNULATA SCHIZONTS AND THE IMMUNITY INDUCED BY THE GELATINPROTECTED, SCHIZONT-PARASITIZED CELL VACCINE
作  者：
Lu Wenshun Li Guandi Lu Wenxing Bai Qi Tian Guangfu Zhao Kaiyuan (Lanzhou Veterinary Research Institute, Chinese Academy of Agricnltural Sciences) Liu Zhengyu Ma Yulin Zhang Zhonghang (The Institute of Zootechnic and Veterinary Medicine of Ningxia Hui Autonomous Region)
摘  要：
The medium used in these studies was prepared by addition of folie acid to BLS and lactalbumin hydrolysate. The number of lymphoid cells parasitized by the schizonts of Theileria annulata was increased by more than five times within four days, and more than 95% of the passaged lymphoid cells were parasitized. Gelatin had good preserving effect on the vitality of the cultured cells.Vaccines prepared with the cells of the 5th, 9th, 15th and 17th generations caused no obvious untoward clinical reactions in the vaccinated cattle, and cells of the 6th and 9th generations could stimulate the production of gametocytes in the inoculated animals, while those of the 15th generation and upwards could not. Challenge trials by attacking the vaccinated cattle with 100 ticks (Hyalomma detritum) per animal showed that the protection rates were 66.67,83.3 and 83.3—100% with vaccines containing 1×10~5, 5×10~5—10×10~5, and 5×10~6 cells per dose respectively. The duration of storage of the vaccine was two months at 4°—6℃。 Protection was initiated 20 days after vaccination and may last for more than 82 days.From 1974 to 1976, 3, 215 yellow cattle and dairy cows were vaccinated in field experiments in two epizoatic areas in Ningxia Hui Autonomous Region and Xinjiang uighur Autonomous Region. The vaccine was found safe to use, and the protection rates were 98.01 and 85.76% respectively. Cattle may be protected to survive the epizoatic seasons of theileriasis safely.

相似文章

导出引用
计量
文章访问数: 3
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46