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Position: Home > Articles > Effects of Fructus lycii on Growth of Cervical Cancer Hela Cells Food and Nutrition in China 2013,19 (03) 69-71

枸杞子对人宫颈癌Hela细胞生长的影响

作  者：
欧嘉文;何冬梅;李江滨;余涌珠;郑若洋;陈婉嫦;杨丽梅
单  位：
广东医学院医学检验学院;惠州市中医医院妇产科
关键词：
枸杞子;宫颈癌;Hela细胞;细胞生长
摘  要：
目的:体外实验探讨枸杞子对人宫颈癌细胞(Hela)生长的影响。方法:不同浓度的枸杞子水煎液处理Hela细胞,采用细胞贴壁率实验测定细胞的粘附能力,通过细胞划痕实验观察细胞的迁移能力、利用细胞增殖实验检测细胞增殖抑制率,运用流式细胞术分析细胞周期变化。结果:与对照组相比,5.0mg/mL枸杞子水煎液处理后的Hela细胞贴壁率减少(P<0.01),细胞迁移能力减弱(P<0.01),对Hela细胞有显著的抑制作用,抑制率最高达84.88%(P<0.01),且呈现时效和量效关系;流式细胞术结果表明,枸杞子水煎液组对Hela细胞有一定的S期阻滞作用。结论:枸杞子能有效抑制人宫颈癌细胞的增殖、粘附和迁移能力,并改变细胞的周期分布。
译  名：
Effects of Fructus lycii on Growth of Cervical Cancer Hela Cells
作  者：
OU Jia-wen 1,HE dong-mei2,LI Jiang-bin1,YU Yong-zhu1, ZHENG Ruo-yang1,CHEN Wan-chang1,YANG Li-mei1(1 School of Medical Laboratory,Guangdong Medical College,Dongguan523808,China; 2 Department of Obstetrics and Gynecology,Huizhou Chinese Medicine Hospital,Huizhou 516001,China)
关键词：
Fructus lycii;cervical cancer;Hela cell;cell growth
摘  要：
Objective To investigate the effects of Fructus lycii on the in-vitro growth of cervical cancer Hela cells.Method Hela cells were treated with different concentrations of the decoction of Fructus lycii,and its effects on the adhesion and migration ability of Hela cells were determined by the cell-matrix adhesion assay and wound healing.The cell proliferation assay and Flow Cytometer were used to detect the inhibitory effects and the change of growing cycle.Result Compared with control groups,the adhesive ratio of Hela cells treated with 5.0mg/mL Fructus lycii reduced(P<0.01)and the migration ability of Hela cells became weaker(P<0.01).The cell proliferation assay showed that Fructus lycii inhibited the growth of Hela cells effectively and the inhibited radio reached up to 84.88%(P<0.01),and the inhibit function of Fructus lycii to Hela cells showed an obvious relationships of time-effectiveness and does-effectiveness.The result of flow cytometry showed that Fructus Lycii could block the cell cycle at S phase.Conclusion Fructus lycii could inhibit the ability of adhesion,migration and proliferation of Hela cells,and it also changed the cycle of the Hela cells.

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