当前位置: 首页 > 文章 > 猪繁殖与呼吸综合征病毒美洲型RT-LAMP检测方法建立 中国动物检疫 2015 (2) 57-62
Position: Home > Articles > Development of an RT-LAMP Assay for Detection of American Type Porcine Reproductive and Respiratory Syndrome Virus(PRRSV) China Animal Health Inspection 2015 (2) 57-62

猪繁殖与呼吸综合征病毒美洲型RT-LAMP检测方法建立

作  者:
蒲静;汪琳;尹羿;柏亚铎;任彤;张伟;高志强;乌日古玛拉;时培蛟;肖春芳
单  位:
北京出入境检验检疫局
关键词:
猪繁殖与呼吸综合征病毒;美洲型;RT-LAMP
摘  要:
本研究针对猪繁殖与呼吸综合征病毒美洲型ORF6基因的6个区域,利用2对引物建立检测猪繁殖与呼吸综合征病毒的RT-LAMP快速检测方法,该方法具有高灵敏度,高特异性的优点,在2小时左右即可得出结果,其灵敏度与PRRSV荧光RT-PCR检测方法相近,高于普通RT-PCR检测方法。将灭活的猪繁殖与呼吸综合征病毒美洲型培养物作10倍系列稀释,结果显示建立的荧光RT-PCR的检测极限可达10-7,RT-PCR检测方法的检测极限为10-5,RT-LAMP检测方法达到10-8,表明建立的RT-LAMP检测方法非常灵敏,可用于检测猪组织样本中的美洲型猪繁殖与呼吸综合征病毒。
译  名:
Development of an RT-LAMP Assay for Detection of American Type Porcine Reproductive and Respiratory Syndrome Virus(PRRSV)
作  者:
Pu Jing;Wang Ling;Ying Yi;Bo Yaduo;Zhang Wei;Gao Zhiqiang;Wurigumala;Shi Peijiao;Xiao Chunfang;Beijing Exit-Entry Inspection and Quarantine Bureau;
关键词:
porcine reproductive and respiratory syndrome;;American-type virus;;RT-LAMP
摘  要:
Two pairs of primers targeting six regions of American-type porcine reproductive and respiratory syndrome virus ORF6 gene,and RT-LAMP assay was established for the rapid detection of porcine reproductive and respiratory virus with high sensitivity and specificity. It could be finished in about 2 hours. The sensitivity of the assay was similar to PRRSV fluorescent RT-PCR,and higher than the normal RT-PCR. The inactivated porcine reproductive and respiratory syndrome virus culture was serially diluted in 10-folds,and tested with PRRSV fluorescent RT-PCR,RTLAMP assay and normal RT-PCR. The result showed that the fluorescence RT-PCR detection limit was up to 10-7,the normal RT-PCR detection limit was 10-5,and the RT-LAMP detection limit was 10-8,indicating RT-LAMP assay was very sensitive. The assay could be used for rapid detection of the American-type porcine reproductive and respiratory syndrome virus in various pig tissue samples.

相似文章

计量
文章访问数: 4
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊