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Position: Home > Articles > Cloning and Sequence Analysis of gyrB Gene of Fluoroquinolones-resistant Salmonella Isolated from Chickens Journal of Northeast Agricultural University(English Edition) 2005,12 (1)

Cloning and Sequence Analysis of gyrB Gene of Fluoroquinolones-resistant Salmonella Isolated from Chickens

作者：

LIU Fang-ping;TONG Heng-min;LI Chang-wen

单位：

;College of Veterinary Medicine. Northeast Agricultural University, Harbin Heilongjiang 150030, PRC

关键词：

fluoroquinolone;salmonella;gyrb gene;cloning;sequence analysis

摘要：

Nine strains resistant to five fluoroquinolones (Ciprofloxacin, Ofloxacin, Enrofloxacin, Danofloxacin, Sarafloxacin) were isolated from clinical samples and extracted the chromosomal DNA of these strains. Designed primers to amplify the Quinolone-resistance-determining region (QRDR) of gyrB gene, then the PCR products were cloned and the sequence was analyzed. In comparison with the standarded strain NCTC5776, no mutation was found in the QRDR of gyrB gene of all resistant strains. The result indicated that the QRDR of gyrB has little relationship with fluoroquinolone resistance to salmonella.

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Cloning and Sequence Analysis of gyrB Gene of Fluoroquinolones-resistant Salmonella Isolated from Chickens

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东北农业大学学报(英文版)

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