Position: Home > Articles > Effect of F_(2α) receptor agonist fluprostenol on mRNA expression of TGF-α,TGF-β3,FGF-2,LIF and GM-CSF in bovine oviduct epithelial cell
Chinese Journal of Veterinary Science
2017
(2)
345-351
前列腺素F_(2α)受体激动剂fluprostenol对奶牛输卵管上皮细胞合成分泌细胞因子表达的影响
作 者：
张莹;曹金山;张楠
单 位：
内蒙古农业大学兽医学院
关键词：
前列腺素F2α受体激动剂;fluprostenol;奶牛输卵管上皮细胞;TGF-α;TGF-β3;FGF-2;LIF;GM-CSF
摘 要：
为验证前列腺素类化合物对输卵管分泌细胞因子有调控作用,以前列腺素F2α受体激动剂fluprostenol对奶牛输卵管上皮细胞合成分泌细胞因子TGF-α、TGF-β3、FGF-2、LIF和GM-CSF的调控机制为研究对象,采用实时荧光定量PCR技术检测吲哚美辛(10-5 mol/L)作用不同时间(2,4,8,16,24,48h)对奶牛输卵管上皮细胞TGF-α、TGF-β3、FGF-2、LIF和GM-CSF mRNA表达的影响。结果显示,fluprostenol对奶牛输卵管上皮细胞中TGF-α和FGF-2mRNA有正调控的作用,均在4h时mRNA的表达量达到峰值;fluprostenol对奶牛输卵管上皮细胞中LIF和GMCSF mRNA有双重调控作用,均是先短暂显著促进(P<0.05)其mRNA的表达,之后表现为极显著抑制(P<0.01),并均在2h时达到表达量峰值;fluprostenol对奶牛输卵管上皮细胞中TGF-β3mRNA起到正调控作用,作用48h时与空白对照组相比极显著升高(P<0.01),达到峰值。
译 名：
Effect of F_(2α) receptor agonist fluprostenol on mRNA expression of TGF-α,TGF-β3,FGF-2,LIF and GM-CSF in bovine oviduct epithelial cell
作 者：
ZHANG Ying;CAO Jin-shan;ZHANG Nan;College of Veterinary Medicine,Inner Mongolia Agricultural University Inner;
关键词：
prastaglandin F_(2α) receptor agonist;;fluprostenol;;oviduct epithelial cells;;TGF-α;;TGF-β3;;FGF-2;;LIF;;GM-CSF
摘 要：
To reveal the regulation of the prostaglandin F_(2α) receptor agonist fluprostenol on mRNA expression of TGF-α,TGF-β3,FGF-2,LIF and GM-CSF in bovine oviduct epithelial cell.Real-time PCR were used to detect TGF-α,TGF-β3,FGF-2,LIF and GM-CSF mRNA expression in bovine oviduct epithelial cells after(fluprostenol,indomethacin(10-5 mol/L))treatment at indicated time points(2,4,8,16,24,48h).Fluprostenol promoted the mRNA expression levels of TGF-αand FGF-2in bovine oviductal epithelial cells,and both reached the maximum at 4h.Fluprostenol had dual regulation to LIF and GM-CSF,and both reached the maximum at 2hsignificantly increased compared with blank group(P<0.05),and then,the expression of these cytokine was extremely decreased(P<0.01).Fluprostenol promoted the mRNA expression levels of TGF-β3,reached the maximum at 48 h,significantly increased(P<0.01),compared with blank group.
导出引用
计量
文章访问数: 10
HTML全文浏览量: 0
PDF下载量: 0
所属期刊
