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Position: Home > Articles > Development of NYDL-2000 alliance for fast measurement of erucic acid and glucosinolate in rapeseed Chinese Journal of Oil Crop Sciences 2003,25 (2)

油菜芥酸硫甙定量速测仪研制及应用

作  者:
李培武;孟子园;张文;杨湄;仝乘风;李光明;周霞;吴渝;谢立华;汪雪芳;丁小霞;中岛一成;押川雄孝;安达武史
单  位:
中国农业科学院油料作物研究所;湖北省油菜籽生产技术开发现地实证调查日方专家组;中国农业科学院仪器设施技术服务中心;湖北省油菜籽生产技术开发现地实证调查日方专家组 湖北武汉430062;中国农业科学院计划财务局;农业部油料及制品质量监督检验测试中心
关键词:
油菜;芥酸;硫甙;速测仪
摘  要:
基于硫代葡萄糖甙与特异外源酶和显色剂反应生成有色产物与菜籽硫甙含量的关系以及芥酸形成浊度与芥酸含量的相关关系,研制了NYDL-2000油菜芥酸硫甙定量速测仪,芥酸测量范围0.5%~8.0%,硫甙测量范围10.0~60.0μmol/g。芥酸和硫甙的速测结果误差分别为±0.5%和±4.0μmol/g,符合国家标准和国际标准。在农技推广及粮油生产基点现场应用结果表明,具有测定快速、操作简单、测试费用低等特点。
译  名:
Development of NYDL-2000 alliance for fast measurement of erucic acid and glucosinolate in rapeseed
作  者:
LI Pei-wu1,MENG Zi-yuan2,ZHANG Wen1,YANG Mei1,TONG Cheng-feng2, LI Guang-ming1, ZHOU Xia3,WU Yu1,XIE Li-hua1,WANG Xue-fang1, DING Xiao-xia1,NAKAJIMA KAZHUSHIGE4,OSHIKAWA YUKO4, ADACHI TAKESHI4 (1.Oil Crops Research Institute,CAAS , Wuhan 430062, China; 2.Instrument Service Center,CAAS, Beijing, 100081,China; 3.Financial Bureau, CAAS, Beijing 100081, China; 4.JICA Project Wuhan Office, Wuhan 430062, China)
关键词:
Rapeseed; Erucic acid; Glucosinolate; Determination alliance
摘  要:
This paper reports development and application of NYDL-2000 alliance for fast determination of erucic acid and total glucosinolates in rapeseed. Based on exterior enzymatic hydrolysis of glucosinolates and turbidimetry of erucic acid, an alliance instrument characterized by small sized, portable, accurate and fast analysis for both erucic acid and total glucosinolates concentration was developed. The detecting scope for erucic acid was from 0.5% to 8.0% with standard error ±0.5%.And detecting scope for glucosinolates was from 10.0 to 60.0μmol/g with standard error ±4.0μmol/g. Comparison of the results by NYDL-2000 alliance and the standard methods with GC and HPLC showed good correlation. NYDL-2000 alliance was spread and used in double-low rapeseed production area in Hubei, Hunan, Henan, Sichuan, Jiangsu and Jiangxi provinces from 2001 to 2002. It is expected to be a new fast and efficient method for double-low rapeseed quality testing in China.

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