Position: Home > Articles > Extraction of Isoflavone Aglycones from Soybean Hypocotyl with C_(18) Column Chromatography
FOOD SCIENCE
2004,25
(4)
136-138
C_(18)柱层析法提取大豆胚轴中异黄酮苷元
作 者：
全吉淑;尹学哲;工藤重光
单 位：
延边大学医学院生物化学教研室;日本东北食效科学研究所;延边大学医学院附属医院
关键词：
C_(18)柱层析法;豆科;异黄酮苷元
摘 要：
从大豆胚轴提取总糖苷后,采用C18反相柱层析法分离大豆异黄酮糖苷,再将其水解制备成异黄酮苷元。产品经薄层色谱法(TLC)和高效液相色谱法(HPLC)分析异黄酮苷元纯度和含量。
译 名：
Extraction of Isoflavone Aglycones from Soybean Hypocotyl with C_(18) Column Chromatography
作 者：
QUAN Ji-shu1, YIN Xue-zhe2, Kudou Shigemitsu3(Department of Biochemistry, Yanbian University College of Medicine, Yanji 133000, China)
关键词：
C_(18) column chromatography;Legumes;isoflavone aglycones
摘 要：
Total glycosides were extracted from soybean hypocotyl ,Where isoflavone glycosides were isolated with C18reversed phase column chromatography. Finally isoflavone aglycones were prepared by hydrolysis of isoflavone glycosides.The purity and contents of isoflavone aglycones were assayed with thin layer chromatograghy (TLC) and high-performance liquidchromatography (HPLC).
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