当前位置: 首页 > 文章 > 基因C型鸭甲肝病毒VP1基因的克隆及原核表达 中国预防兽医学报 2012,34 (9) 740-742
Position: Home > Articles > Prokaryotic expression of duck hepatitis A virus genotype C VP1 gene Chinese Journal of Preventive Veterinary Medicine 2012,34 (9) 740-742

基因C型鸭甲肝病毒VP1基因的克隆及原核表达

作  者:
皮晋魁;聂培婷;黄秋雪;岳华;张焕容
单  位:
西南民族大学生命科学与技术学院
关键词:
基因C型鸭甲肝病毒;VP1基因;原核表达
摘  要:
为原核表达基因C型鸭甲肝病毒(DHAV-C)VP1重组蛋白,本研究通过设计套式PCR引物,RT-PCR扩增DHAV-C VP1全基因,约为720 bp,将其亚克隆至pET-32a(+)载体中构建重组表达质粒pET-VP1。将其转化E.coli Rosetta(DE3)中,经IPTG诱导表达了约47 ku的重组蛋白。Western blot分析表明,该重组蛋白可以与兔抗DHAV-C阳性血清发生特异性反应,具有良好的反应原性。
译  名:
Prokaryotic expression of duck hepatitis A virus genotype C VP1 gene
作  者:
PI Jin-kui1,NIE Pei-ting1,HUANG Qiu-xue1,YUE Hua1,2,ZHANG Huan-rong1,2(1.College of Life Science and Technology,Southwest University for Nationalities,Chengdu 610041,China; 2.Key Laboratory of Veterinary Medicine of Universities in Sichuan Province,Chengdu 610041,China)
关键词:
duck hepatitis A virus genotype C;VP1 gene;prokaryotic expression
摘  要:
To express duck hepatitis A virus genotype C(DHAV-C) VP1 gene,Nested-PCR primers were designed to amplify VP1 gene from DHAV-C strain by RT-PCR and cloned into pET-32a(+) for expressing in E.coli recombinant VP1was highly expressed at 8 hour under 1 mM IPTG induction.SDS-PAGE showed the relative molecular weight of the VP1 recombinant protein was about 47 ku,and western blot analysis showed that the VP1 recombinant protein reacted with rabbit anti DHAV-C.

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