Position: Home > Articles > Development of a double-antibody sandwich enzyme linked immunosorbent assay for detection of Cronobacter muytjensii in milk powder
Science and Technology of Food Industry
2012
(1)
335-337
检测乳粉中克罗诺杆菌属穆汀斯克罗诺杆菌的双抗夹心ELISA方法的研究
作 者：
石曼;生威;杜欣军;王帅;杨丽;余桂春;郭柏雪;王硕
单 位：
食品营养与安全教育部重点实验室天津科技大学食品工程与生物技术学院
关键词：
穆汀斯克罗诺杆菌;双抗夹心ELISA;全脂乳粉;检测
摘 要：
获得了抗穆汀斯克罗诺杆菌的多克隆抗体,抗体只对穆汀斯克罗诺杆菌菌株有特异性识别,而对非穆汀斯克罗诺杆菌无交叉反应。利用所得到的抗体,建立了一种双抗夹心ELISA检测方法,该方法对纯培养穆汀斯克罗诺杆菌菌液检出限为105cfu/mL;经过17h增菌,全脂乳粉染菌样品中的穆汀斯克罗诺杆菌的检出限为0.1cfu/g。该方法为快速检测乳粉中穆汀斯克罗诺杆菌的污染奠定了基础。
译 名：
Development of a double-antibody sandwich enzyme linked immunosorbent assay for detection of Cronobacter muytjensii in milk powder
作 者：
SHI Man,SHENG Wei,DU Xin-jun,WANG Shuai,YANG Li,YU Gui-chun,GUO Bai-xue,WANG Shuo (Key Laboratory of Food Nutrition and Safety,Ministry of Education,College of Food Engineering and Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China)
关键词：
C.muytjensii;double-antibody sandwich ELISA;whole milk powder;detect
摘 要：
The polyclonal antibody(PcAb) for Cronobacter muytjensii was available by intravenous injection with New Zealand rabbit with C.muytjensii ATCC51329 strains,a double-antibody sandwich enzyme-linked immunosorbent assay(ELISA) was developed.The detection limits were 105cfu/mL in pure culture of C.muytjensii ATCC51329 and without any cross reaction with the non-C.muytjensii strains.With 17h enrichment procedure,C.muytjensii recovered well from inoculated whole milk powder at levels of 0.1cfu/g.The results demonstrated that the double-antibody sandwich ELISA was a sensitive and specific method for detecting C.muytjensii in dairy products.
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