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当前位置: 首页 > 文章 > 高效液相色谱法同时测定香灰菌发酵液中L-苯丙氨酸和2-苯基乙醇 食品工业科技 2020,41 (14) 210-215
Position: Home > Articles > Simultaneous Detection of L-phenylalanine and 2-phenylethanol in Hypoxylon sp.Fermented Liquid by HPLC Science and Technology of Food Industry 2020,41 (14) 210-215

高效液相色谱法同时测定香灰菌发酵液中L-苯丙氨酸和2-苯基乙醇

作  者：
杨立志;林隆基;宋燕娇;杨娟;邓优锦;江玉姬
单  位：
福建农林大学;福建农林大学食品科学学院
关键词：
香灰菌;发酵液;L-苯丙氨酸;2-苯基乙醇;同时测定;高效液相色谱
摘  要：
为了检测香灰菌发酵液中L-苯丙氨酸的利用情况和2-苯基乙醇的生成量,建立了一种利用HPLC外标法同时检测香灰菌发酵液中L-苯丙氨酸和2-苯基乙醇的方法。方法色谱条件为:色谱柱:C_(18)(5μm,250 mm×4.6 mm);柱温30℃;进样量10μL;流速0.7 mL/min;DAD检测器,检测波长258 nm;流动相A:0.6%乙酸水溶液;流动相B:甲醇。结果表明:两种物质的浓度分别在1～5和1.02～5.1 g/L范围内与峰面积有良好的线性关系,相关系数r均大于0.999,L-苯丙氨酸检出限和定量限分别为1.023和3.409 mg/L,2-苯基乙醇检出限和定量限分别为0.567和1.890 mg/L,L-苯丙氨酸加标回收率为99.16%～101.58%,RSD(n=6)为0.67%,2-苯基乙醇的加标回收率为98.53%～101.06%,RSD(n=6)为1.22%。该方法操作简便快速、准确度和灵敏度高,可满足对香灰菌发酵液中L-苯丙氨酸和2-苯基乙醇风味物质的同时检测。
译  名：
Simultaneous Detection of L-phenylalanine and 2-phenylethanol in Hypoxylon sp.Fermented Liquid by HPLC
作  者：
YANG Li-zhi;LIN Long-ji;SONG Yan-jiao;YANG Juan;DENG You-jin;JIANG Yu-ji;College of Food Science,Fujian Agriculture and Forestry Uneiversity;Mycological Research Center,Fujian Agriculture and Forestry Uneiversity;
关键词：
Hypoxylon sp.;;fermented liquid;;L-phenylalanine;;2-phenylethanol;;simultaneous determination;;HPLC
摘  要：
In order to simultaneously detect the usage of L-phenylalanine and the production of 2-phenyl-ethanol in Hypoxylon sp. fermented liquid,an HPLC external standard method was developed. The method chromatographic conditions were as follows:Chromatographic column:C_(18)(5 μm,250 mm×4.6 mm),Column temperature 30 ℃,the injection volume 10 μL,flow rate 0.7 mL/min,DAD detector,detection wavelength 258 nm,mobile phase A 0.6% acetic acid solution,mobile phase B methanol.The results indicated that good linearities between concentration and peak area were exist for L-phenylalanine and 2-phenylethanol with correlation coefficients greater than 0.999 in the range of 1～5 and 1.02～5.1 g/L,respectively.The detection limit and the quantitative limit of L-phenylalanine were 1.023 and 3.409 mg/L,respectively. The detection limit and the quantitative limit of 2-phenylethanol were 0.567 and 1.890 mg/L,respectively. The spiked recoveries of L-phenylalanine were in the range of 99.16%～101.58% with relative standard deviations(n=6)of 0.67%. The spiked recoveries of 2-phenylethanol were in the range of 98.53%～101.06% with relative standard deviations(n=6)of 1.22%.The method was simple width high accuracy and sensitivity. It was suitable for simultaneously determining the L-phenylalanine and 2-phenylethanol in the Hypoxylon sp. fermented liquid.

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