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Position: Home > Articles > Comparative studies on mitochondrial DNA from triploid crucian carp and its parents (allotetraploid hybrids of crucian carp and common carp,♂and Carassius auratus cuvieri,♀) Journal of Fishery Sciences of China 2001,8 (1) 1-5

三倍体湘云鲫及其亲本线粒体DNA的比较研究

作  者：
黎双飞;刘少军;张轩杰;罗琛;周工建;刘筠
单  位：
湖南师范大学生命科学学院
关键词：
白鲫;异源四倍体鲫鲤;三倍体湘云鲫;线粒体DNA;限制性内切酶;遗传距离
摘  要：
用人工选育的异源四倍体鲫鲤 (♂ )与白鲫 (♀ )杂交获得具有明显生长优势的三倍体湘云鲫。采用差速离心和核酸酶处理等方法 ,从三倍体、白鲫和异源四倍体鲫鲤肝组织中提取线粒体DNA ,并用 9种限制性内切酶进行单酶酶切分析。经琼脂糖凝胶电泳后计算出各酶切片段的大小 ,测得三倍体、白鲫和异源四倍体鲫鲤mtDNA的分子大小分别为 16 .2 4kb、16 .6 0kb和 16 .2 0kb。根据各单倍型间的酶切片段共享度 ,估算出 3个群体间的遗传距离 ,说明了mtDNA母系遗传的特性。
译  名：
Comparative studies on mitochondrial DNA from triploid crucian carp and its parents (allotetraploid hybrids of crucian carp and common carp,♂and Carassius auratus cuvieri,♀)
作  者：
LI Shuang fei, LIU Shao jun, ZHANG Xuan jie, LUO Chen, ZHOU Gong jian, LIU Yun (College of Life Science, Hunan Normal University, Changsha 41008, China)
关键词：
Carassius auratus cuvieri; allotetraploid crucian carp; triploid crucian carp; mitochondrial DNA(mtDNA); restriction endonuclease; genetic distance
摘  要：
The triploid crucian carp, with obvious advantage in growth, was obtained by crossing allotetraploid hybrids(♂) from offsprings(F 3～F 9)of red crucian carp (Carassius auratus red var.)(♂) ×common carp (Cyprinus carpio L.) (♀), and C.auratus cuvieri (♀). The mitochonodrial DNA (mtDNA) of liver were isolated from the triploids, Japanese crucian carp (C. auratus cuvieri) and allotetraploid hybrids by means of density gradient centrifugation, DNase I and RNase digestion, and were digested by 9 kinds of restriction endonucleases. By analysis of agrose gel electrophoresis, the sizes of mtDNA fragments were estimated that the mtDNA sizes of triploids, C. auratuscuvieri and allotetraploid hybrids were 16.24×10 3, 16.60×10 3 and 16.30×10 3 bp, respectively, and the genetic distances among mtDNA haploidy types in the 3 populations were calculated according to the numbers of common fragments that they had the matrilinear inheritence character of mtDNA.

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