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Position: Home > Articles > Study on Genome DNA Damage of Aging Seeds of Elymus sibiricus L.Based on ISSR Marker Chinese Journal of Grassland 2015,37 (2) 63-67

基于ISSR标记的老芒麦老化种子基因组DNA损伤的研究

作  者:
周国栋;李志勇;李鸿雁;扈顺;师文贵;刘磊;黄帆
单  位:
中国农业科学院草原研究所;内蒙古农牧科学院;临夏州农业科学院
关键词:
老芒麦;种子老化;ISSR
摘  要:
从人工老化后的不同发芽率水平的老芒麦干种子中提取基因组DNA,检测表明其可用于老芒麦种质资源的ISSR分析。ISSR标记分析表明,种子老化引起的老芒麦干种子基因组DNA分子片段的变化主要集中在750~2000bp和250~500bp范围,主要表现为DNA大分子片段的消失或颜色逐渐变浅,某些特异DNA片段出现,且其丢失和增加是随机的。
译  名:
Study on Genome DNA Damage of Aging Seeds of Elymus sibiricus L.Based on ISSR Marker
作  者:
ZHOU Guo-dong;LI Zhi-yong;LI Hong-yan;HU Shun;SHI Wen-gui;LIU Lei;HUANG Fan;Academy of Agricultural Sciences of Linxia;Grassland Research Institute,Chinese Academy of Agriculture Science;Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences;
关键词:
Elymus sibiricus L;;Seed aging;;ISSR
摘  要:
Dry seed genomic DNA was extracted after artificial aging to be used for ISSR-PCR genetic integrity analysis of Elymus sibiricus L.germplasm resources.The changes of dry seed genomic DNA fragments of Elymus sibiricus L.caused by seed aging mainly concentrated in 750~2000bp and 250~500bp and showed that macromolecular fragments of DNA disappeared and color became shallow gradually.Some specific DNA fragments appeared,and the lost and increase of genomic DNA fragments were random.

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