当前位置: 首页 > 文章 > 加拿大披碱草×肥披碱草杂种F_1幼穗组培再生体系的研究 安徽农业科学 2008,36 (6) 2235-2237
Position: Home > Articles > Studies on the Tissue Culture and Regeneration System of Hybrid F_1 of Elymus canadensis×E. excelsus Journal of Anhui Agricultural Sciences 2008,36 (6) 2235-2237

加拿大披碱草×肥披碱草杂种F_1幼穗组培再生体系的研究

作  者:
李小雷;于卓;马艳红;谢芳利;李造哲;周亚星
单  位:
内蒙古农业大学农学院
关键词:
加拿大披碱草;肥披碱草;种间杂种F1;愈伤组织诱导;植株再生
摘  要:
[目的]筛选适宜加拿大披碱草×肥披碱草种间杂种F1愈伤组织诱导、幼苗分化和生根的最适培养基。[方法]以加拿大披碱草×肥披碱草种间杂种F1幼穗为外植体,以MS为基本培养基,添加不同的激素和营养物,探讨了2,4-D、6-BA、NAA与IBA4种激素不同浓度对杂种F1愈伤组织的诱导、分化及生根的影响,建立了组培再生体系。[结果]激素2,4-D对杂种F1愈伤组织诱导起决定性作用。适宜杂种F1愈伤组织诱导的最佳培养基为:MS+CH300.0 mg/L+2,4-D2.0 mg/L,诱导率达100%;适宜幼苗分化的最佳培养基为:MS+CH300.0 mg/L+6-BA2.0 mg/L+NAA0.4 mg/L,分化率达73.3%;适宜生根的最佳培养基为:MS+CH300.0 mg/L+IBA0.5 mg/L,生根率达86.7%;组培苗经过炼苗移栽得到了完整植株。[结论]杂种F1组培再生体系的建立为新种质的保存奠定了基础。2,4-D适宜浓度为2.0 mg/L。
译  名:
Studies on the Tissue Culture and Regeneration System of Hybrid F_1 of Elymus canadensis×E. excelsus
作  者:
LI Xiao-lei et al(Agronomy College,Inner Mongolia Agricultural University,Hohhot,Inner Mongolia 010019)
关键词:
Elymus canadensis;E.excelsus;Interspecific hybrid F1;Callus tissue induction;Plant regeneration
摘  要:
[Objective] The aim of research was to screen the optimal medium of callus induction,plantlets differentiation and rooting of hybrid F1 of Elymus Canadensis×E.excelsus.[Method] With the young spikes of hybrid F1 of E.Canadensis×E.excelsus as explant and MS as basic medium,the different hormones and nutrients were added to the MS medium to discuss the effect of 4 kinds of hormones including 2,4-D,6-BA,NAA and IBA.on the callus induction,plantlet differentiation and rooting of hybrid F1 and the plant regeneration system was established.[Result] The hormone 2,4-D played a decisive role in the callus-inducing of hybrid F1.The optimal medium of callus induction of hybrid F1 was MS + CH 300.0 mg/L + 2,4-D 2.0 mg/L with the induction rate of 100%.The optimal medium of plantlets differentiation was MS + CH 300.0 mg/L +6-BA 2.0 mg/L + NAA 0.4 mg/L with the differentiation rate of 73.3%.The optimal medium of rooting was MS + CH 300.0 mg/L+IBA 0.5 mg/L with the rooting rate of 86.7%,and the regenerated plant was obtained after acclimatization and transplanting.[Conclusion] The establishment of tissue culture and regeneration system laid a foundation for preservation of germplasm resource.

相似文章

计量
文章访问数: 15
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊