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当前位置: 首页 > 文章 > 藻红蛋白衍生比率荧光法检测消毒液中邻苯二甲醛 上海海洋大学学报 2019 (6) 994-1001
Position: Home > Articles > R-phycoerythrin-derivatized ratio probe for fluorimetric determination of o-phthalaldehyde in disinfectant Journal of Shanghai Ocean University 2019 (6) 994-1001

藻红蛋白衍生比率荧光法检测消毒液中邻苯二甲醛

作  者：
任宁娜;贾敏;吴继魁
单  位：
上海海洋大学食品学院;上海水产品加工及贮藏工程技术中心;农业农村部水产品贮藏保鲜质量安全风险评估实验室
关键词：
邻苯二甲醛;R-藻红蛋白;衍生化反应;比率荧光;含量测定;消毒液
摘  要：
基于R-藻红蛋白(R-PE)与邻苯二甲醛(OPA)的衍生化反应,构建一种新型检测邻苯二甲醛的荧光比率型探针。R-藻红蛋白经十二烷基硫酸钠(SDS)去折叠后裸露出伯氨基团,之后在β巯基乙醇存在的碱性环境中,与邻苯二甲醛相互反应衍生出一种蓝色荧光的异吲哚类化合物。在该体系中,随着邻苯二甲醛浓度的增加,R-藻红蛋白本身的荧光信号逐渐减弱,衍生产物的蓝色荧光信号逐渐增强,从而构成了比率型荧光探针。结果表明:在pH 10.5的碳酸盐缓冲条件下,探针的最佳检测时间为3～6 min,线性检测范围为5～100μg/mL,相关系数R~2为0.992,最低检测限为1.7μg/mL。该探针应用于市售洗手液样品测定,加标回收结果满意。
译  名：
R-phycoerythrin-derivatized ratio probe for fluorimetric determination of o-phthalaldehyde in disinfectant
作  者：
REN Ningna;JIA Min;WU Jikui;College of Food Science and Technology, Shanghai Ocean University;Shanghai Engineering Research Center of Aquatic Product Processing & Preservation;Laboratory of Quality and Safety Risk Assessment for Aquatic Product on Storage and Preservation, Ministry of Agriculture and Rural Affairs;
关键词：
o-phthalaldehyde;;R-phycoerythrin;;derivatization reaction;;ratio fluorescence;;content determination;;disinfectant
摘  要：
Based on the derivatization of R-phycoerythrin(R-PE) with o-phthalaldehyde(OPA), a novel fluorescence ratio probe for the detection of o-phthalaldehyde was constructed. The primary amino groups of R-phycoerythrin were exposed after unfolding by sodium dodecyl sulfate(SDS). In the alkaline environment of β-mercaptoethanol, a blue fluorescent isoindole compound could be derived by reacting with o-phthalaldehyde. In this probe, the fluorescence signal of R-phycoerythrin gradually weakened with the increase of the concentration of o-phthalaldehyde, whereas the blue fluorescence signal was gradually enhanced, and a strong-weak fluorescence change constituted a ratio fluorescence probe. The results show that under the carbonate buffer condition of pH 10.5, the optimal detection time of the probe is from 3 to 6 minutes, the linear detection range is from 5 to 100 μg/mL, the correlation coefficient is 0.992, and the limit of detection is 1.7 μg/mL. The proposed fluorescent probe can sensitively detect the OPA in disinfectant.

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