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当前位置: 首页 > 文章 > 猪繁殖与呼吸综合征病毒S-1株ORF5和ORF7基因的克隆及变异分析 上海农业学报 2008,24 (2) 40-45
Position: Home > Articles > Clone and variation analysis of the ORF5 and ORF7 genes of Porcine Reproductive and Respiratory Syndrome Virus(PRRSV) S-1 strain Acta Agriculturae Shanghai 2008,24 (2) 40-45

猪繁殖与呼吸综合征病毒S-1株ORF5和ORF7基因的克隆及变异分析

作  者：
张春玲;张婉华;臧克伟;王英;蒋凤英;邹勇;李春华;何锡忠
单  位：
上海市农业科学院畜牧兽医研究所;山东农业大学
关键词：
猪繁殖与呼吸综合征病毒;ORF5基因;ORF7基因;变异分析
摘  要：
PRRSV S-1株经Marc-145复苏后,通过RT-PCR扩增了ORF5和ORF7基因。利用酶切位点将2个基因分别连接到原核表达载体PET-32C中,筛选阳性克隆送测序,序列分析表明:PRRSV S-1株ORF5基因变异性较高,与VR-2332毒株和CH-1 a毒株的同源性分别是:核苷酸同源性为91%和89%,氨基酸同源性是87%和85%;ORF7基因相对保守,核苷酸的同源性分别是97%和94%,氨基酸的同源性分别是95%和93%。
译  名：
Clone and variation analysis of the ORF5 and ORF7 genes of Porcine Reproductive and Respiratory Syndrome Virus(PRRSV) S-1 strain
作  者：
ZHANG Chun-ling1,ZHANG Wan-hua1,ZANG Kewei2,WANG Ying1,JIANG Feng-ying1,ZOU Yong1*,LI Chu-hua1,HE Xi-zhong1 (1Animal Husbandry and Veterinary Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai 201106,China;2College of Veterinary Medicine,Shandong Agricultural University,Taian 271018,China)
关键词：
Porcine reproductive and respiratory syndrome virus;ORF5 gene;ORF7 gene;Variation analysis
摘  要：
After the revival of PRRSV S-1 strain by Marc-145 cells,the ORF5 and ORF7 genes of PRRSV S-1 strain were amplified by RT-PCR and cloned directly into the prokaryotic expression vector pET-32.The sequence analysis showed that the variability of ORF5 gene was relatively high and the homology between the PRRSV S-1 and VR-2332 strains and between the PRRSV S-1 and CH-1a strains was respectively 91% and 89% in nucleotide and 87% and 85% in amino acid.The ORF7 gene was relatively conservative,and compared with the VR-2332 and Ch-1a strains the homology was respectively 97% and 94% in nucleotide and 95% and 93% in amino acid.

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