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当前位置: 首页 > 文章 > 苹果抗炭疽菌叶枯病基因SNP和InDel标记的HRM筛选 园艺学报 2017,44 (2) 215-222
Position: Home > Articles > Screening of SNP and InDel Markers to Glomerella Leaf Spot Resistance Gene Locus in Apple Using HRM Technology Acta Horticulturae Sinica 2017,44 (2) 215-222

苹果抗炭疽菌叶枯病基因SNP和InDel标记的HRM筛选

作  者：
刘源霞;兰进好;柏素花;孙晓红;刘春晓;张玉刚;戴洪义
单  位：
青岛农业大学园艺学院;青岛农业大学生命科学学院;青岛农业大学农学与植物保护学院
关键词：
苹果;高分辨率熔解曲线;炭疽菌叶枯病;SNP标记;InDel标记;精细定位
摘  要：
以207株‘金冠’与‘富士’苹果的F1杂交分离群体实生树为试材,挑选抗炭疽菌叶枯病基因R_(gls)位点区域内的,第15条染色体上,位于SSR标记S0304673和S0405127之间500 kb物理距离内,由全基因组重测序技术所获得的与抗该病相关的10个SNP及10个InDel标记,通过高分辨率熔解曲线(High Resolution Melting,HRM)技术筛选出与基因R_(gls)位点紧密连锁的2个SNP及4个InDel标记。通过对重组个体的基因型及表型分析,发现标记InDel4227和InDel4254表现出与R_(gls)基因位点共分离,将基因R_(gls)精细定位于标记InDel4199和SNP4299之间100 kb的物理距离内。对该基因位点两侧4.1~4.3Mb距离内的基因进行统计分析,表明在该区段内存在40个有功能注释的基因,涉及到细胞组成、分子功能和生物过程方面共19个GO分类。
译  名：
Screening of SNP and InDel Markers to Glomerella Leaf Spot Resistance Gene Locus in Apple Using HRM Technology
作  者：
LIU Yuanxia;LAN Jinhao;BAI Suhua;SUN Xiaohong;LIU Chunxiao;ZHANG Yugang;DAI Hongyi;Qingdao Key Laboratory of Genetic Improvement and Breeding in Horticultural Plants,College of Horticulture,Qingdao Agricultural University;College of Agronomy and Crop Protection,Qingdao Agricultural University;College of Life Sciences,Qingdao Agricultural University;
关键词：
apple;;HRM;;Glomerella leaf spot;;SNP marker;;InDel marker;;fine mapping
摘  要：
In present study,10 SNP and 10 InDel markers associated with Glomerella leaf spot(GLS)resistance in the region of R_(gls),on apple linkage group(LG)15,in a region of 500 kb between S0304673 and S0405127 markers,obtained by using the whole genome sequencing technology were tested in the population of 207 F1 individuals derived from a cross between‘Golden Delicious' and‘Fuji'apples by High Resolution Melting(HRM). Two SNP and 4 InDel markers tightly linked to the R_(gls) locus were screened. Through analyzing the genotypes and phenotypes of the recombinant individuals,the R_(gls) locus was fine mapped within a distance of 100 kb between InDel4199 and SNP4299 markers on the‘Golden Delicious' genome sequence and was co-segregated with InDel4227 and InDel4254 markers. Forty genes were predicted within the region of 4.1–4.3 Mb flanked the R_(gls) locus,including cellular component,molecular function and biological process in a total of 19 GO classifications.

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