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当前位置: 首页 > 文章 > 木薯华南8号及其四倍体块根淀粉代谢相关基因表达分析 南方农业学报 2018,49 (8) 1484-1489
Position: Home > Articles > Analysis of gene expression associated with starch metabolism on cassava cv. SC8 tuberous roots and its tetraploid Journal of Southern Agriculture 2018,49 (8) 1484-1489

木薯华南8号及其四倍体块根淀粉代谢相关基因表达分析

作  者：
安飞飞;冷青云;李开绵;陈松笔
关键词：
木薯;四倍体;淀粉含量;淀粉代谢;基因表达;实时荧光定量PCR(qRT-PCR)
摘  要：
【目的】分析木薯华南8号(SC8)及其四倍体块根中淀粉代谢相关基因表达情况,为揭示多倍化影响木薯块根淀粉代谢的分子机制及木薯块根中淀粉的合成与分解途径提供理论参考。【方法】利用秋水仙素诱导木薯腋芽以获得SC8四倍体,采用实时荧光定量PCR(qRT-PCR)对蔗糖磷酸合酶(SPS)、ADP葡萄糖焦磷酸化酶(AGPase)、可溶性淀粉合成酶(SSS)、颗粒结合合成酶(GBSS)、分支酶(SBE)、α-淀粉酶(α-amylase)、β-淀粉酶(β-amylase)和淀粉磷酸化酶(SP)等基因的表达情况进行分析。【结果】经秋水仙素诱导获得的SC8四倍体植株叶片较SC8二倍体厚,且叶色深绿。SC8四倍体块根的干物率和粗淀粉含量均较SC8二倍体显著降低(P<0.05,下同),而支链淀粉和直链淀粉比例均未发生显著变化(P>0.05,下同)。与SC8二倍体相比,MeSPS2、Meα-amylase和Meβ-amylase基因的表达量显著上调(P<0.05,下同),表明SC8四倍体块根中淀粉的分解能力较SC8二倍体强;MeAGPase、MeSSS1、MeSP1和MeSP2基因的表达量显著上调,表明SC8四倍体块根淀粉的合成能力较SC8二倍体弱;MeGBSSI和MeSBE1基因的表达量未发生显著变化,表明SC8四倍体块根中直链淀粉和支链淀粉的合成能力与SC8二倍体无明显差异。【结论】利用秋水仙素诱导获得的SC8四倍体块根淀粉合成能力降低和淀粉分解能力升高是导致其块根中粗淀粉含量显著降低的主要原因。
译  名：
Analysis of gene expression associated with starch metabolism on cassava cv. SC8 tuberous roots and its tetraploid
作  者：
AN Fei-fei;LENG Qing-yun;LI Kai-mian;CHEN Song-bi;Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Ministry of Agriculture and Rural Affairs for Germplasm Resources Conservation and Utilization of Cassava;
关键词：
cassava;;tetraploid;;starch content;;starch metabolism;;gene expression;;real-time fluorescence quantitative PCR(qRT-PCR)
摘  要：
【Objective】In order to elaborate the molecular mechanism for the effects of polyploification on starch metabolism in cassava and also lay a theoretical basis for studying the synthesis and decomposition process of starch in tuberous roots,genes expressions that related to starch metabolism were analyzed in cassava SC8 and its tetraploid.【Method】SC8 tetraploid was obtained by inducing cassava axillary buds with colchicine. Real-time fluorescence quantitative PCR(RT-PCR)was used in this study to analyze the expression of the genes associated with starch metabolism,including sucrose phosphate synthase(SPS),ADP-glucose pyrophosphorylase(AGPase),soluble starch synthase(SSS),granulebound starch synthase(GBSS),starch branching enzyme(SBE),α-amylase,β-amylase and starch phosphorylase(SP).【Result】Flow cytometry result showed that SC8 tetraploid induced by colchicine had thicker leaves and the color was dark green,its dry matter content and crude starch content were significantly decreased than that of SC8 diploid(P<0.05,the same below),while the proportion of amylopectin and amylose did not showed significantly difference(P>0.05, the same below). The relatively expression level of MeSPS2,Meα-amylase and Meβ-amylase were unregulated,it indicated that the starch decomposition ability of SC8 tetraploid was stronger than SC8 diploid. The relatively expression level of MeAGPase,MeSSS1,MeSP1 and MeSP2 were downregulated significantly,it showed that the starch synthesis ability of SC8 tetraploid was weaker than SC8 diploid. While the relative expression level of MeGBSSI,MeSBE1 were not significantly changed, indicating that there was no significant difference in amylose and amylopectin synthesis between SC8 diploid and its tetraploid.【Conclusion】The results indicates that the starch synthesis ability of SC8 tetraploid induced by colchicine is decreased,and starch decomposition ability is increased,this may be the main reason for the significant decline of crude starch content in SC8 tetraploid root tuber.

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