中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 种子老化对扁蓿豆种质遗传完整性变化的影响 中国草地学报 2010,32 (6) 52-57
Position: Home > Articles > Effect of Seed Aging on Changes of Genetic Integrity in Melilotoides ruthenica Germplasm Chinese Journal of Grassland 2010,32 (6) 52-57

种子老化对扁蓿豆种质遗传完整性变化的影响

作  者：
王小丽;李志勇;李鸿雁;师文贵
单  位：
中国农业科学院草原研究所/农业部沙尔沁牧草资源重点野外科学观测试验站;甘肃农业大学草业学院/草业生态系统教育部重点实验室
关键词：
扁蓿豆;种子老化;遗传完整性;SSR;种质资源保存
摘  要：
采用SSR分子标记对经老化处理的不同发芽率水平扁蓿豆种质进行遗传完整性分析,结果表明:(1)用10个有效引物对4个不同发芽率种质进行PCR扩增,共检测到25个等位基因,每个位点的等位基因数在1～4之间,平均为2.5个;(2)老化处理群体的多态性位点百分率、等位基因数、有效等位基因数、基因多样性指数、Shannon指数等遗传参数与对照相比都有所下降,表明老化处理后群体内的遗传多样性低于对照;发芽率为20%时,等位基因数呈显著性差异,说明扁蓿豆种质更新发芽率临界值在20%～40%之间;(3)SSR分子标记可以作为老化扁蓿豆种子遗传完整性变化的检测方法,对于异质种质资源材料,低的发芽率更新标准不利于种质资源遗传完整性的保持。
译  名：
Effect of Seed Aging on Changes of Genetic Integrity in Melilotoides ruthenica Germplasm
作  者：
WANG Xiao-li1,2,LI Zhi-yong1,LI Hong-yan2,SHI Weng-gui2(1.College of Grassland Science,Gansu Agricultural University/Key Laboratory of Grassland Ecosystem,Ministry of Education,Lanzhou 730070,China;2.Grassland Research Institute of Chinese Academy of Agricultural Science/Key Scientific Field Observation and Test Station of Forage Resources in Shaerqin,Department of Agriculture,Hohhot 010010,China)
关键词：
Melilotoides ruthenica;Seed aging;Genetic integrity;SSR;Germplasm conservation
摘  要：
The effects of artificial aging on genetic integrity of Melilotoides ruthenica germplasm populations in different germination level were studied based on SSR markers.The results showed that 25 alleles amplified by PCR were detected with ten primers,number of alleles per locus was 1～4,mean number of alleles was 2.5;the percentage of polymorphic bands,the number of alleles(Na),effective number of alleles(Ne),Nei's gene diversity index(H) and Shannon's information index(I) were lower in artificially aged population than in high germination(CK) population based on SSR;the number of alleles was lower than that of CK population when germination rate of the population was 20%,and the difference was significant(P<0.05),which indicated that regeneration germination was 20%～40%;SSR molecular markers can be used as a method with which genetic integrity of Melilotoides ruthenica could be detected;a low germination percentage standard for regeneration is disadvantageous for maintaining the genetic integrity of germplasm resource.

相似文章

导出引用
计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46