当前位置: 首页 > 文章 > 蓝鳃太阳鱼与绿色太阳鱼细胞色素b(Cyt b)基因序列片段分析 浙江海洋学院学报(自然科学版) 2009,28 (4) 389-392+405
Position: Home > Articles > Sequences Analysis of Mitochondrial Cytochrome b Gene Fragment on Lepomis macrochirus and Lepomis cyanellus Journal of Zhejiang Ocean University(Natural Science) 2009,28 (4) 389-392+405

蓝鳃太阳鱼与绿色太阳鱼细胞色素b(Cyt b)基因序列片段分析

作  者:
李行先;郭水荣;冯晓宇;谢楠;许宝青
单  位:
杭州市农业科学研究院
关键词:
蓝鳃太阳鱼;绿色太阳鱼;细胞色素b
摘  要:
用酚/氯仿提取法提取了蓝鳃太阳鱼、绿色太阳鱼肌肉组织的总DNA,利用特异引物进行PCR扩增,得到1 119bp Cyt b基因片段,纯化后送上海生工测序。所得序列用Mega3.1软件分析,并结合GenBank中其余9种太阳鲈属鱼类Cyt b基因序列用NJ法构建太阳鲈属鱼类系统发育树。结果表明:(1)蓝鳃太阳鱼与绿色太阳鱼序列相似度为82.3%,变异位点198个,平均转换/颠换比为2.1;(2)蓝鳃太阳鱼与L.humilis亲缘关系较近,绿色太阳鱼与L.symmetricus亲缘关系较近,蓝鳃太阳鱼与绿色太阳鱼遗传距离为0.206。
译  名:
Sequences Analysis of Mitochondrial Cytochrome b Gene Fragment on Lepomis macrochirus and Lepomis cyanellus
作  者:
LI Xing-xian,GUO Shui-rong,FENG Xiao-yu,et al(Fishery Research Institute,Hangzhou Academy of Agricultural Sciences,Hangzhou 310024,China)
关键词:
Lepomis macrochirus;Lepomis cyanellus;cytochrome b
摘  要:
The DNA sequences of 1 119 bp mitochondrial cytochrome b gene fragment of Lepomis macrochirus and Lepomis cyanellus were analyzed with Mega 3.1.The molecular phylogenetic tree was based on the sequences of 11 Lepomis species with neighbor-joiningmethod(NJ) respectively.The result were as follows:(1) The sequences identity matrix got 82.3%,and the substituted position between the two sequences got 198,with the transition/transversion ratio of 2.1.(2)There were cluster relationships between Lepomis macrochirus and L.humilis,Lepomis cyanellus and L.symmetricus respectively.The genetic distance between Lepomis macrochirus and Lepomis cyanellus got 0.206.

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