作 者：
吴高峰;黄占旺;刘宛玲;牛丽亚;王素贞;黄永平
单 位：
江西农业大学食品科学与工程学院江西省天然产物与功能食品重点实验室
摘 要：
本实验旨在探究纳豆冻干粉(natto lyophilized powder,NLP)对昆明小鼠免疫功能及其细胞因子分泌的影响。选取体质健康昆明小鼠128只,随机分为8组:空白对照组(C组)、调节组(R0组和RN1、RN2、RN3组)4组、预防组(P0、PN组)2组、模型组(M组)。除C组外,其他各组灌胃3 d抗生素溶液,之后R0组灌胃生理盐水,RN1~RN3组灌胃不同剂量的NLP溶液;预防组每天继续灌胃抗生素溶液,8 h后P0、PN组分别灌胃生理盐水和NLP溶液;M组灌胃抗生素溶液。30 d后测定免疫指标及其细胞因子分泌量的变化情况。结果表明:与M组相比,低剂量的NLP能够极显著增加小鼠的脾脏指数和胸腺指数(P<0.01),RN1、RN2、RN3组小鼠血清中白细胞介素-2(interleukin-2,IL-2)、IL-10、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、干扰素-γ(interferon-γ,IFN-γ)的分泌量极显著增加(P<0.01),同时,RN1、RN2、RN3组均能够极显著增加小鼠血清中诱导型一氧化氮合酶(inducible nitric oxide synthase,i NOS)活力(P<0.01),RN1、RN3组小鼠血清中白蛋白、球蛋白的含量和二者比值(白球比)及NO水平显著或极显著增加(P<0.05或P<0.01)。C组、调节组、预防组小鼠的血清溶血素水平较对照组均极显著升高(P<0.01)。RN1、RN2、RN3、PN组较M组均能够极显著增强小鼠腹腔巨噬细胞吞噬作用(P<0.01)。与C组相比,RN1、RN2、RN3组小鼠的半数溶血值显著或极显著升高(P<0.05或P<0.01)。RN3和PN组可极显著增加巨噬细胞吞噬百分率、鸡红细胞的吞噬数(P<0.01)。结果表明NLP具有免疫增强作用和增加细胞因子分泌的作用。
译 名：
Effect of Lyophilized Natto Powder on Antibiotics-Mediated Immunomodulation and Cytokine Secretion in Mice
作 者：
WU Gaofeng;HUANG Zhanwang;LIU Wanling;NIU Liya;WANG Suzhen;HUANG Yongping;Jiangxi Key Laboratory of Natural Product and Functional Food,College of Food Science and Engineering,Jiangxi Agricultural University;
关键词：
natto lyophilized powder(NLP);;antibiotics;;immunomodulatory effect
摘 要：
The effect of natto lyophilized powder(NLP) on antibiotics-mediated immunomodulation and cytokine secretion in SPF mice was explored in the present study. Totally 128 healthy mice were selected and divided randomly into 8 groups: control group(C), four regulation groups(RN0, 1, 2 and 3), two prevention groups(P0 and PN), and model group(M). The mice in all groups except C were administered with antibiotic solution for 3 days. Thereafter, the mice from group R0 were gavaged with normal saline, while those from group RN1, 2 and 3 were given NLP solution at three different dosages, respectively. The mice from the two prevention groups were continuously daily administered with antibiotic solution; 8 hour later, groups P0 and PN were respectively lavaged with normal saline and NLP solution. The mice in group M were simply given antibiotic solution during the entire administration period of 30 days. Immunomodulatory effects of NLP in mice and its effect on cytokine secretion were determined. Results showed that low-dose NLP resulted in a significant elevation in spleen and thymus indices in mice compared with the model group(P < 0.01). The production of serum IL-2, IL-10, TNF-alpha, and IFN-gamma extremely significantly increased in groups RN1, RN2 and RN3 compared with the model group(P < 0.01). In addition, the mice from the three prevention groups showed an extremely significant increase in inducible nitric oxide synthase(i NOS) activity in serum(P < 0.01), and serum albumin and globulin concentrations and their ratio as well as serum NO level were significantly or extremely significantly higher in the mice from groups RN1 and RN3(P < 0.05 or P < 0.01). The serum hemolysin levels in mice from the control, regulation and prevention groups were significantly higher than those in the group N(P < 0.01). The phagocytic capacity of peritoneal macrophages from mice in groups RN1, RN2, RN3 and PN was significantly higher than that of group M. Compared with the control group, the half value of hemolysis(HC50) in groups RN1, RN2 and RN3 was significantly or extremely significantly increased(P < 0.05 or P < 0.01). Groups RN3 and PN exhibited an extremely significant increase in the percentage of macrophages engaged in phagocytosis and the percent phagocytosis of chicken red blood cells(P < 0.01). Therefore, NLP can enhance immune function and increase the secretion of cytokines.
