Position: Home > Articles > Expression analysis of EgPIP1;3 gene in cut eustoma flowers by semi-quantitative RT-PCR
Journal of Zhongkai University of Agriculture and Engineering
2012,25
(1)
6-10
半定量RT-PCR法分析水孔蛋白基因EgPIP1;3在洋桔梗切花中的表达
作 者：
丁岳炼;李红梅;林燕飞;何生根;黄新敏
单 位：
仲恺农业工程学院生命科学学院;仲恺农业工程学院园艺园林学院
关键词：
洋桔梗(Eustoma grandiforum);切花;水孔蛋白;半定量RT-PCR;基因表达
摘 要：
为研究水孔蛋白基因EgPIP1 3在洋桔梗(Eustoma grandiforum)采后切花中的表达特点,采用肌动蛋白(β-actin)基因为内参,以洋桔梗花瓣cDNA为模板,通过对PCR体系循环数及退火温度的优化,建立了一个稳定、特异的半定量反转录PCR(Reverse transcription PCR,RT-PCR)体系.运用此体系检测了EgPIP1 3在洋桔梗切花中的表达.结果表明,EgPIP1 3基因在洋桔梗切花的茎、叶、萼、花瓣、雄蕊和雌蕊中均有表达,但表达量存在明显的差异,依次表现为茎>雌蕊>萼>叶>雄蕊>花瓣.
译 名：
Expression analysis of EgPIP1;3 gene in cut eustoma flowers by semi-quantitative RT-PCR
作 者：
DING Yue-lian1,2,LI Hong-mei2*,LIN Yan-fei1,2,HE Sheng-gen2,HUANG Xinmin1,2(1.College of Horticulture and Landscape Architecture,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China;2.College of Life Sciences,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China)
关键词：
eustoma(Eustoma grandiforum);cut flowers;aquaporin;semi-quantitative RT-PCR;gene expression
摘 要：
A stable and specific semi-quantitative reverse transcription PCR(RT-PCR)system for the expression analysis of EgPIP1 3 gene in different organs of cut eustoma(Eustoma grandiforum)flowers was established,using β-actin gene as the internal control,and the cDNA from eustoma petal as the template.Cycle numbers and annealing temperature for this system were optimized.Based on the system,the expression of EgPIP1 3 gene in different tissues of cut eustoma flowers was analyzed.The results showed that the gene could be expressed in all detected tissues of cut eustoma flowers,including stem,leaf,calyx,petal,stamen and pistil.However,there were significant differences in mRNA levels among them.The highest expression level was detected in stems,then immediately followed by pistils,while moderate expression levels were in calyxes,leaves and stamina,and the lowest level was in petals.
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