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当前位置: 首页 > 文章 > 绿盲蝽多聚半乳糖醛酸酶基因的克隆及生物信息学分析 吉林农业大学学报 2014,36 (4) 421-428
Position: Home > Articles > Cloning and Bioinformatics Analysis of Polygalacturonase Gene of Apolygus lucorum(Meyre-Dür) Journal of Jilin Agricultural University 2014,36 (4) 421-428

绿盲蝽多聚半乳糖醛酸酶基因的克隆及生物信息学分析

作  者：
王林;贾秋磊;姜秀萍
单  位：
大连理工大学生命科学与技术学院
关键词：
绿盲蝽;多聚半乳糖醛酸酶;基因克隆;生物信息学
摘  要：
从绿盲蝽(Apolygus lucorum)成虫中克隆得到1个多聚半乳糖醛酸酶(Polygalacturonase,PG)基因ALPG(GenBank登录号JQ288100.1),其碱基序列为1 050 bp,编码350个氨基酸。获得的该碱基序列与美国豆荚草盲蝽(Lygus hesperus)PG具有很高的同源性,碱基序列一致性为93%。该基因编码蛋白AL-PG有氨基端疏水信号肽序列(MKFTIYALGLLVAVASA)。预测的成熟肽相对分子质量为35.9 kD,等电点为8.89。AL-PG二级结构由大量的β-折叠、β-转角和无规则卷曲组成,而α-螺旋的数量非常少。AL-PG三级结构是右手平行的β螺旋结构,包含4组β折叠,PB1,PB2a,PB2b和PB3。催化氨基酸残基Asp153,Asp173和Asp174在底物结合裂缝的底部,底物结合裂缝入口的最小距离为8.4。
译  名：
Cloning and Bioinformatics Analysis of Polygalacturonase Gene of Apolygus lucorum(Meyre-Dür)
作  者：
WANG Lin;JIA Qiu-lei;JIANG Xiu-ping;College of Bioscience and Biotechnology,Dalian University of Technology;
关键词：
Apolygus lucorum;;polygalacturonase;;gene cloning;;bioinformatics
摘  要：
A polygalacturonase gene AL-PG was cloned from the adults of the Apolygus lucorum(GenBank accession number: JQ288100.1),which contained a 1 050 bp nucleotide sequence encoding 350 amino acids. The obtained sequence had relatively high homology with the sequence of Lygus hesperus PG by sharing 93% sequence identity. AL-PG protein encoded by this gene had a hydrophobic N-terminal signal sequence(MKFTIYALGLLVAVASA). The calculated molecular mass of the mature protein was 35. 9 kD with an estimated pI of 8. 89. The secondary structure of AL-PG consisted of many β-sheets,β-turns and random coils,however,there was a small amount of α-helices. The tertiary structure of AL-PG had a right-handed parallel β-helical structure,consisting of four β-sheets,PB1,PB2a,PB2b and PB3. The catalytic amino acid residues,Asp153,Asp173and Asp174,were at the bottom of substrate binding cleft. The smallest distance at the entry of the substrate binding cleft was 8. 4

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