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当前位置: 首页 > 文章 > 盐度对日本囊对虾仔虾生长发育和Na~+-K~+-ATPase活力的影响 水生生物学报 2005,29 (6)
Position: Home > Articles > THE EFFECTS OF SALINITY ON DEVELOPMENT AND Na~+-K~+-ATPase ACTIVITY OF MARSUPENAEUS JAPONICUS POSTLARVAE Acta Hydrobiologica Sinica 2005,29 (6)

盐度对日本囊对虾仔虾生长发育和Na~+-K~+-ATPase活力的影响

作  者：
潘鲁青;栾治华
单  位：
中国海洋大学海水养殖教育部重点实验室
关键词：
盐度;地下卤水;日本对虾仔虾;生长发育;Na+-K+-ATPase
摘  要：
研究了盐度对日本囊对虾仔虾(Marsupenaeus Japonicus)的生长发育和Na+-K+-ATPase活力的影响。结果表明,盐度对日本囊对虾仔虾存活率、增重率和Na+-K+-ATPase活力的影响显著(P<0.05)。随着向低盐度变化的增加,仔虾的存活率和增重率明显下降,而向高盐度变化无显著差异;在盐度变化48h内,随着盐度变化的增加仔虾Na+-K+-ATPase活力变化增大,各处理组仔虾Na+-K+-ATPase活力随着取样时间的增加呈峰值变化,至24h时低盐度处理组酶活力达到最大值,而高盐度处理组达到最小值:至48h~72h时,不同盐度下仔虾Na+-K+-ATPase活力趋于稳定,而且盐度越低酶活力越大。由此表明日本囊对虾仔虾在低盐度地下卤水中的适宜盐度为20‰—24‰。
译  名：
THE EFFECTS OF SALINITY ON DEVELOPMENT AND Na~+-K~+-ATPase ACTIVITY OF MARSUPENAEUS JAPONICUS POSTLARVAE
作  者：
PAN Lu-Qing and LUAN Zhi-Hua(The Key Laboratory of Mariculture,Ministry of Education,Ocean University of China,Qingdao 266003)
关键词：
Salinity;Underground brine water;Growth;Na~+-K~+-ATPase
摘  要：
The effects of salinity on development and Na~+-K~+-ATPase activity of Marsupenaeus japonicus postlarve were studied in order to discuss the osmoregulation mechanism of Marsupenaeus japonicus in this paper.It analyzed the feasibility that Marsupenaeus japonicus was cultured in saline-alkali area with view of osmotic physiology and provided the scientific basis of culturing Marsupenaeus japonicus using underground brine water.In the test,underground brine water was diluted to the desired salinity levels of 12,16,20 and 24 with yellow river fresh water.Na~+/K~+ in the water was adjusted to 30 with Potassium chloride(AR).And then Shrimps acclimatized in water of salinity 20 were put into these treatments.Groups of seawater served as the control groups.All trials were conducted in triplicates.In development test,survival rate and weight gain of Marsupenaeus japonicus postlarve was counted after 72 hours.Count the daily body weight gain of the larvae as follows:Daily body weight gain(μg/ind·d)=final body weight-primal body weight Experiment time·number of postlarvesIn Na~+-K~+-ATPase activity test,the shrimps were sampled at 0h,3h,6h,12h,24h,36h,48h,60h and 72h.The samples were preserved in-20℃.The Na~+-K~+-ATPase was determined by comparison between two media,one of which containing its specific inhibitor,ouabain(3mM),without K~+ according to the method of Esmann.The activity was expressed in micromoles of(inorganic) phosphate(Pi) per hr and per mg of protein.Protein was determined according to Bradford's method with bovin serum albumin(Sigma) as reference.All the results were given as average of three replicates data and analyzed by ANOVA and(Duncan's) Multiple Range Test.Salinity had significant effects on survival rate,daily body weight gain and Na~+-K~+-ATPase activity of Marsupenaeus japonicus postlarve(p<0.05=).Survival rate and growth rate of postlarve decreased significantly with salinity decrease,while had no significant difference with salinity increased.The Na~+-K~+-ATPase activities of seawater groups had no significant difference from groups of salinity of 30.And along with the salinity change increasing within 48h,the change of Na~+-K~+-ATPase activities of postlarves increased,activites of each treated group presented wave change,and was up to maximum in hyposaline-treated groups but up to minimum in hypersaline-treated groups till 24h.The Na~+-K~+-ATPase activity of postlarves tended to be stable from 48h to 72h,and was higher while salinity was lower.From above,it suggested that the feasible salinity of Marsupenaeus japonicus postlarve in hyposaline water was 20—24.

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