译 名：
STUDIES ON OPTIMAL COPPER SUPPLEMENTATION IN THE DIET FOR THE JUVENILE FLOUNDER， PARALICHTHYS OLIVACEUS
作 者：
WEI Wanquan，LI Aijie，LI Deshang （Fisheries College，Ocean University of Qingdao，Qingdao 266003）
关键词：
juvenile flounder (Paralichthys olivaceus); diet; copper; zinc-superoxide dismutase (Cu,Zn-SOD)
摘 要：
： Altogether five experimental diets were respectively formulated by adding copper 0, 0.5, 1.5, 3.0 and 5.0mg/kg to the basal diet which contained 4.16mgCu/kg. The juvenile flounder, Paralichthys olivaceus, with initial mean weight 32.85~35.20g, was fed with one of the above diets for 31 days. The results showed that at the end of the experiment both survival and weight gain rate were similar in fish fed different diets, and the mean values were 92.50~100.00% and 96.21~106.21%, respectively. The copper concentration in the liver and muscle increased significantly with the increased copper supplementation, and at every copper level of the diet, liver copper concentration was much higher than that of the muscle, thus indicating a high copper accumulation ability of the liver. The activity of copper and zinc-superoxide dismutase (Cu,Zn- SOD, EC1.15.1.1) in fish liver rose rapidly with the supplementation of copper to basal diet, and reached an approximate plateau when 1.5~5.0mg/kg copper were added. According to fish growth and enzyme activity, in the studied Practical diet for juveile flounder, the optimal copper supplementation was 1.5mg/kg, and the total dietary copper was about 5.6mg/kg.
