当前位置: 首页 > 文章 > 普鲁兰酶产生菌的筛选及诱变技术研究 吉林农业大学学报 2007,29 (3) 343-346
Position: Home > Articles > Study on Screening and Mutation of Pullulanase Producing Bacteria Journal of Jilin Agricultural University 2007,29 (3) 343-346

普鲁兰酶产生菌的筛选及诱变技术研究

作  者:
闵伟红;刘艳;沈淑杰;任惠杰;张锦玉
单  位:
长春职业技术学院食品与生物技术分院;吉林农业大学食品工程学院
关键词:
芽孢杆菌;筛选;诱变;普鲁兰酶
摘  要:
从土壤中筛选出1株普鲁兰酶产生菌,经过紫外线诱变及紫外线—甲基磺酸乙酯复合诱变后,从大量突变株中筛选出1株稳定产普鲁兰酶的菌株。诱变育种最佳条件为紫外线照射时间2 min,菌浓度104;甲基磺酸乙酯(体积分数10 mL/L)处理50 min。酶活力由出发菌的2.06 U/mL提高到9.37 U/mL,比国内报道的最高值8.8 U/mL高出0.57 U/mL。
译  名:
Study on Screening and Mutation of Pullulanase Producing Bacteria
作  者:
MIN Wei-hong1,LIU Yan1,SHEN Shu-jie1,REN Hui-jie2,ZHANG Jin-yu2(1.College of Food Engineering,Jilin Agricultural University,Changchun 130118,China;2.College of Food and Biotechnology,Changchun College of Vocational Technology,Changchun 130033,China)
关键词:
bacillus;screening;mutation;pullulanase
摘  要:
A productive and stable pullulanase bacillus was screened from numerous mutated strains after one strain of bacillus separated from soil had been induced to go through mutation under ultraviolet ray and compound mutation under ultraviolet ray-methyl sulfonic acid ethyl ester.The optimum conditions are: ultraviolet irradiation time 2 min,bacterium density 104,ethylmethane sulfonate density 10 mL/L,treatment for 50 min,and enzymatic activity improved from 2.06 U/mL to 9.37 U/mL.The result exceeds the highest domestic report by 0.57 U/mL.

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