Position: Home > Articles > Activity Determination of Litchi Water-soluble Polysaccharide (LCWSP) as α-Glucosidase Inhibitor
FOOD SCIENCE
2013,34
(13)
175-179
荔枝水溶性多糖作为α-葡萄糖苷酶抑制剂的活性测定
作 者：
张钟;吴文婷;王萍;张玲
单 位：
广东石油化工学院化学与生命科学学院
关键词：
荔枝水溶性多糖(LCWSP);α-葡萄糖苷酶抑制剂;活性
摘 要：
对荔枝果肉水溶性多糖(LCWSP)对α-葡萄糖苷酶的活性影响进行研究,以黑叶荔枝作为材料,通过热水浸提、醇沉、离心得到LCWSP提取液,经过脱色,Sevag法去蛋白,透析除去离子后,将LCWSP提取液加入α-葡萄糖苷酶溶液中,利用4-硝基酚-α-D-吡喃葡萄糖苷(PNPG)法测定α-葡萄糖苷酶的活性。结果表明:LCWSP对α-葡萄糖苷酶具有明显抑制作用,且其抑制效果是阿卡波糖的1.25倍;LCWSP抑制作用的大小会受到温度、pH值、时间及LCWSP质量浓度的影响,在单因素试验的基础上,通过正交试验确定LCWSP对α-葡萄糖苷酶抑制的最佳反应温度为30℃、pH值为6.8、时间25min、抑制剂质量浓度为80mg/mL。说明LCWSP对α-葡萄糖苷酶的抑制作用比较明显,可以作为α-葡萄糖苷酶抑制剂;LCWSP可能具有降血糖的作用。
译 名：
Activity Determination of Litchi Water-soluble Polysaccharide (LCWSP) as α-Glucosidase Inhibitor
作 者：
ZHANG Zhong,WU Wen-ting,WANG Ping,ZHANG Ling(College of Chemistry and Life Science,Guangdong University of Petrochemical Technology,Maoming 525000,China)
关键词：
LCWSP;α-gucosidase inhibitor;activity
摘 要：
Litchi water-soluble polysaccharide(LCWSP) was extracted from black leaf litchi through hot water extraction,alcohol precipitation,centrifugation,and then bleached and deproteinized by Sevag’s method.After dialysis,LCWSP was added to α-glucosidase solution and PNPG method was used to determine the activity of the enzyme.The results showed that LCWSP had an obvious inhibition effect on α-glucosidase,which was 1.25 times as high as that of acarbose.Optimal enzyme reaction parameters were determined by orthogonal array design based on single factor analysis.The inhibitory effect of LCWSP was impacted by temperature,pH,time and polysaccharide concentration.The optimal temperature,pH,reaction time and concentration for LCWSP inhibitory effect on α-glucosidase were 30 ℃,6.8,25 min and 80 mg/mL,respectively.LCWSP had an obvious inhibitory effect on α-glucosidase and could be used as an α-glucosidase inhibitor.Meanwhile,LCWSP may have hypoglycemic effect,which provides a new direction for litchi processing and the development of functional foods.
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