单 位：
青海大学高原花卉研究中心青海省园林植物与观赏园艺重点实验室
关键词：
万寿菊(Tagetes erecta L.);雄性不育两用系;SRAP;实时荧光定量PCR
摘 要：
本研究以万寿菊雄性不育两用系2-2、C×40-1(3)、34-1(2)的可育株与不育株叶片为材料,采用SRAP分子标记技术筛选与育性相关的SRAP标记。首先选用24对SRAP引物组合对2-2两用系的可育株和不育株DNA进行扩增,根据差异条带的测序结果设计引物,进行实时荧光定量PCR扩增,计算可育株和不育株中的相对表达量。结果表明,24对引物组合唯有me3/em18组合能在3个两用系中扩增出差异性条带。实时荧光定量PCR扩增结果表明在可育株中目的片段表达量显著高于不育株中的表达量。因此,利用该SRAP标记(S580)可以在万寿菊两用系苗期利用叶片快速鉴定出可育和不育植株。
译 名：
Development of SRAP Molecular Marker for Fertility Related Genes of Marigold
作 者：
Ma Xiuhua;Tang Daocheng;Tang Nan;The Key Laboratory of Landscape Plants of Qinghai Province,Plateau Flower Research Center of Qinghai University;
关键词：
Marigold(Tagetes erecta L.);;Genic male sterile line;;SRAP;;Real-time PCR
摘 要：
In this study, leaves of fertile and sterile plants of marigold male sterile lines 2-2, C×40-1(3), 34-1(2)were used as materials. Sequence-related amplified polymorphism(SRAP) marker was used to screen fertility related markers. 24 pairs of SRAP primer combinations were used to amplify DNA of sterile plants and sterile lines of 2/2 dual purpose lines. Then primers were designed according to the sequencing results of differential bands,and real-time fluorescence quantitative PCR amplification was carried out to calculate the relative expression of fertile and sterile plants. The results showed that sterile and fertile differential band was only obtained by primer combination(me3+em18), real-time PCR amplification indicated that the expression level of the target fragment in fertile plants was significantly higher than sterile plants. Therefore, the selected SRAP marker(S580) can be used to identify quickly fertile and sterile plants in seedling stage of marigold male sterile two lines.
