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Animal Husbandry and Feed Science
2009,1
Detection of mycoplasma contamination in animal cell cultures.
作 者：
Jun Zhao;Wang XingMan;Hu Yong;Jingxian Chen;Mingli Wan
单 位：
Department of Microbiology, Anhui Medical University, Hefei, 230032, Chin;Department of Microbiology, Anhui Medical University, Hefei, 230032, China
关键词：
mycoplasma contamination;staining;detected;cell;samples;dna fluorescenc
摘 要：
[Objective] To develop a rapid efficient method for detecting mycoplasma contamination in cell cultures. [Method] A pair of primers was designed according to two highly conserved nucleotide sequences of the 16S RNA from six kinds of mycoplasma that commonly contaminated cells. Then the mycoplasma contamination of 25 cell samples was detected by PCR and DNA fluorescence staining. [Result] When these cell samples were detected by DNA fluorescence staining, the positive rate and probable positive rate were respectively 24% and 16%. And when they were detected by PCR, the positive rate was 36%. [Conclusion] The PCR method is more sensitive and specific than the DNA fluorescence staining, and combining these two methods is the optimal way to detect mycoplasma contamination in cell cultures.
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