当前位置: 首页 > 文章 > 烟草青枯病拮抗芽孢杆菌的筛选、鉴定及其抑菌活性初探 西北农林科技大学学报(自然科学版) 2014,42 (2) 123-130
Position: Home > Articles > Screening,identification and antifungal activity of antagonistic Bacillus spp.against tobacco bacterial wilt Journal of Northwest A & F University(Natural Science Edition) 2014,42 (2) 123-130

烟草青枯病拮抗芽孢杆菌的筛选、鉴定及其抑菌活性初探

作  者:
刘伟;刘鹏;沈小英;安天赐;成巨龙;安德荣
单  位:
吉林农业大学农学院;中国烟草总公司陕西省烟草研究所;西北农林科技大学植物保护学院/旱区作物逆境生物学国家重点实验室
关键词:
烟草青枯病;拮抗芽孢杆菌;定殖能力;促生作用;防治效果
摘  要:
【目的】从不同烟区健康烟草的根际土壤样品中筛选对烟草青枯病具有较强拮抗作用的芽孢杆菌,为防治烟草青枯病提供生防资源。【方法】随机从福建龙岩、四川德昌、陕西汉中等地健康烟草根际土壤中采集30份土壤样品,采用平板稀释法从中分离芽孢杆菌,以烟草青枯病菌为靶标菌筛选拮抗芽孢杆菌,并通过培养特性、菌体形态、生理生化特征分析及16SrDNA序列分析,对筛选的拮抗芽孢杆菌菌株进行鉴定;采用温室盆栽试验,测定拮抗芽孢杆菌的促生作用、定殖能力和抑菌活性。【结果】从健康烟草根际土壤中分离得到1株抗烟草青枯病菌活性较好的菌株LW-4,经鉴定其为甲基营养型芽孢杆菌Bacillus methylotrophicus。LW-4菌悬液对烟草青枯病菌的防治效果为70.37%,其可定殖于烟草根际土壤,具有良好的定殖能力。LW-4对烟草有明显的促生作用;用饱和度为25%的硫酸铵获得的LW-4抑菌物质对烟草青枯病菌的抑菌活性较高,抑菌圈直径达37.82mm。【结论】菌株LW-4在烟草青枯病防治中具有潜在的利用价值。
译  名:
Screening,identification and antifungal activity of antagonistic Bacillus spp.against tobacco bacterial wilt
作  者:
LIU Wei;LIU Peng;SHEN Xiao-ying;AN Tian-ci;CHENG Ju-long;AN De-rong;College of Plant Protection,Northwest A&F University/State Key Laboratory of Crop Stress Biology for Arid Areas;College of Agronomy,Jilin Agricultural University;Shaanxi Tobacco Research Institute,China National Tobacco Corporation;
关键词:
tobacco bacterial wilt;;antagonistic bacteria;;colonization ability;;growth promoting;;control efficacy
摘  要:
【Objective】Bacillus spp.strains with strong antagonistic effects on tobacco bacterial wilt were selected from healthy tobacco rhizosphere soil samples collected from different tobacco growing areas. 【Method】30samples were randomly collected from places including Longyan,Fujian province,Dechang, Sichuan province,and Hanzhong,Shaanxi province.Plate dilution method was used to separate the strains, and Ralstonia solanacearum was used as the target bacterium.The screened bacillus strains were identified based on morphological,physiological and biochemical characteristics,and 16SrDNA phylogenetic analysis. In addition,bonsai experiment in greenhouse was used to determine the inhibition ability,plant growth-promoting ability and colonization of screened strains.【Result】Strain LW-4with strong antagonistic effect against tobacco bacterial wilt was obtained and identified as Bacillus methylotrophicus.Its control rate of tobacco bacterial wilt was 70.37%.LW-4could colonize on tobacco rhizosphere soil with good colonization ability.LW-4also promoted the growth of tobacco seedlings.The antifungal metabolite extracted from strain LW-4by 25% ammonium sulfate showed the effective inhibition against Ralstonia solanacearum with inhibition zone diameter of up to 37.82mm.【Conclusion】The obtained LW-4strain could be widely used to control tobacco bacterial wilt.

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