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当前位置: 首页 > 文章 > 高增殖性能犬流感CIV-PR8重组病毒的制备 中国预防兽医学报 2012,34 (7) 518-522
Position: Home > Articles > Development of high-replicated canine influenza virus-PR8 reassortant virus Chinese Journal of Preventive Veterinary Medicine 2012,34 (7) 518-522

高增殖性能犬流感CIV-PR8重组病毒的制备

作  者：
周洁文;滕巧泱;闫丽萍;边延峰;张旭;徐大伟;石迎;周雨霞;李泽君
单  位：
内蒙古农业大学兽医学院;天津生机集团股份有限公司;中国农业科学院上海兽医研究所
关键词：
犬流感;重组犬流感病毒;PR8;血凝效价;病毒滴度
摘  要：
为获得高效表达犬流感病毒(CIV)HA和NA抗原的疫苗株,本实验通过反向遗传操作技术将A/canine/Zhejiang/01/2010(H3N2)犬流感病毒(简称ZJCIV)的HA和NA基因与A/Puerto Rico/8/34(H1N1)病毒(PR8)的6个内部基因进行重组,拯救并获得了一株重组病毒CIV-PR8。分别用含100 EID50的CIV-PR8和ZJCIV感染SPF鸡胚,含2×103TCID50的CIV-PR8和ZJCIV感染MDCK细胞,不同时间段取病毒样品测定血凝效价和TCID50值。两者比较表明,CIV-PR8分别在接种后36 h和48 h时血凝效价达到峰值,鸡胚尿液中效价可达210,为野生病毒株的16倍;细胞上清血凝价可达28,为野生病毒株的4倍;病毒增殖曲线表明通过鸡胚扩增和细胞扩增,CIV-PR8的滴度均高于野生病毒株ZJCIV。结果表明,拯救的CIV-PR8增殖能力明显高于野生病毒株ZJCIV,该重组病毒有望成为犬流感疫苗研制的候选病毒株。
译  名：
Development of high-replicated canine influenza virus-PR8 reassortant virus
作  者：
ZHOU Jie-wen1,2,TENG Qiao-yang2,YAN Li-ping2,BIAN Yan-feng3,ZHANG Xu2, XU Da-wei2,SHI Ying2,ZHOU Yu-xia1,LI Ze-jun2(1.College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China;2.Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai 200241,China;3.Tianjin Shengji Group Co.,Ltd.,Tianjin 300384,China)
关键词：
canine influenza;reassortant canine influenza virus;PR8;hemagglutination titer;virus titer
摘  要：
Canine influenza is a highly contagious disease caused by canine influenza virus(CIV).To enhance the expressions of hemagglutination(HA) and neuraminidase(NA) of CIV,a canine-human reassortant influenza viruses CIV-PR8 was generated by reverse genetic technique,which consisted of the segments encoding HA and NA from H3N2 subtype A/canine/ Zhejiang/01/2010(ZJCIV) and 6 internal gene segments from A/Puerto Rico/8/34(H1N1) virus(PR8).The rescued CIV-PR8 was identified by sequence analysis.Virus titrations showed that the HA titer of CIV-PR8 reached peak value of 210 in allantoic fluid within 36 to 48 hours which was 16 times higher than that of ZJCIV,and the HA titer was 28 in culture medium which was 4 times higher than that of ZJCIV when inoculated in chicken embryos with 100 EID50 and in MDCK cells with 2×103 TCID50 of CIV-PR8 and ZJCIV,respectively.The results suggested that reassortant virus of CIV-PR8 was a promising vaccine candidate for canine influenza.

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