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Position: Home > Articles > Enzymatic conversion for γ-aminobutyric acid by Lactococcus lactis Science and Technology of Food Industry 2008 (9) 166-169

乳酸菌细胞转化法制备γ-氨基丁酸的研究

作  者:
傅元欣;张涛;江波;沐万孟
单  位:
食品科学与技术国家重点实验室江南大学
关键词:
乳酸菌;γ-氨基丁酸;细胞转化;谷氨酸脱羧酶
摘  要:
在利用乳酸菌Lactococcus lactis1.009细胞中的谷氨酸脱羧酶转化制备γ-氨基丁酸(GABA)的过程中,探讨了菌体浓度、缓冲液种类及浓度、转化时间、菌龄、磷酸吡哆醛添加量、底物添加方式等因素对GABA产量的影响。结果表明,细胞转化法制备GABA的最佳条件为:菌体浓度10g/L,菌龄14h,缓冲液为0.2mol/L pH4.7的醋酸缓冲液,PLP的添加量为0.1mmol/L。通过多次分批添加谷氨酸,50℃下反应60h后,GABA的积累量可达19.1g/L,转化率为99.9%。
译  名:
Enzymatic conversion for γ-aminobutyric acid by Lactococcus lactis
作  者:
FU Yuan-xin,ZHANG Tao,JIANG Bo*,MU Wan-meng(State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi 214122,China)
关键词:
lLactic acid bacteria;γ-aminobutyric acid;enzymatic conversion;GAD
摘  要:
γ-aminobutyric acid was prepared by L-Glutamate decarboxylase from Lactococcus lactis 1.009.The influence of cell concentration,buffer system,cell age,reaction time and external PLP concentration were studied.The optimum reaction system was 0.2mol/L HAc-NaAc buffer pH4.7,containing 10g/L lactic acid bacteria and 0.1mmol/L pyridoxal phosphate(PLP).Glutamate was added each 10h,after 60h GABA content was 19.1g/L,the conversion of L-Glutamate was 99.9%.

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