Position: Home > Articles > Influence of Buffalo PBMC Activity by Different Resuspension Methods During Washing Process
China Animal Husbandry & Veterinary Medicine
2009,36
(4)
100-102
不同重悬细胞法在洗涤过程中对水牛外周血单个核细胞活率的影响
作 者：
韩忠燕;张宝红;黄海愉;张为宇;黄维义;钟恒禄
单 位：
广西大学动物科学技术学院
关键词：
水牛外周血单个核细胞;重悬细胞法;细胞活率
摘 要：
为了探讨在离心洗涤水牛外周血单个核细胞(peripheral blood mononuclear cell,PBMC)过程中以移液器吹打法和晃动离心管法重悬细胞对其活率的影响。对经密度梯度离心后初步收集到的PBMC,分别用移液器吹打和晃动离心管2种方法重悬细胞,离心洗涤后对细胞活率进行计数。结果发现,移液器吹打法得到的细胞活率为(74.9±2.6)%,而晃动离心管法使细胞活率达到(98.5±0.5)%,差异极显著(P<0.01)。说明在洗涤PBMC时,晃动离心管法重悬细胞能大大提高细胞活率,可为那些对细胞活率要求高的试验提供一个较为有效的方法。
译 名：
Influence of Buffalo PBMC Activity by Different Resuspension Methods During Washing Process
作 者：
HAN Zhong-yan,ZHANG Bao-hong,HUANG Hai-yu,ZHANG Wei-yu,HUANG Wei-yi,ZHONG Heng-lu(College of Animal Science and Technology,Guangxi University,Nanning 530005,China)
关键词：
buffalo peripheral blood mononuclear cell;resuspension methods;cell activity
摘 要：
The peripheral blood mononuclear cell(PMBC) activity of buffalo was comported by different resuspension methods during washing process.The PBMC was separated by density gradient centrifugation,then cell was resuspended by transferpettor-spirting method & centrifuge tube-sloshing method,when cell was washed three times the activity was evaluated.The cell activity by transferpettor-spirting method & centrifuge tube-sloshing method was(74.9±2.6)% and(98.5±0.5)% respectively,the difference of cell activity was significant(P<0.01).The activity of PBMC was higher by centrifuge tube-sloshing,which could provide an useful method for further study that requiring higher cell activity.
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