Position: Home > Articles > Efficacy of Bronopol against Fungus Saprolegnia Infection in Fertilized Eggs of Rainbow Trout(Oncorhynchus mykiss)
Chinese Journal of Fisheries
2015,28
(5)
23-26
溴硝基丙二醇抑制虹鳟受精卵水霉菌的效果
作 者：
孙鹏;王炳谦;谷伟;户国
单 位：
中国水产科学研究院黑龙江水产研究所
关键词：
溴硝基丙二醇;虹鳟;受精卵;水霉菌
摘 要：
在泉水水温(4.5±1.3)℃下,将虹鳟Oncorhynchus mykiss受精到发眼期的胚胎放入桶式孵化器中孵化,施用7个浓度梯度的溴硝基丙二醇(2-溴-2硝基-1,3-丙二醇;分子式:C3H8Br NO4;以下简称溴硝醇):0mg/L(对照组)、100mg/L、300mg/L、500mg/L、700mg/L、900mg/L,和1100mg/L,研究其抑制虹鳟受精卵水霉病的效果,以浓度为1000 mg/L的甲醛和双氧水作比较。结果表明:在浸泡15min的情况下,随着溴硝醇浓度的增加,发眼率逐渐增加,浓度为300mg/L时发眼率(78%)最高,高于此浓度则随着浓度的递增而逐渐降低;孵化率随着溴硝醇浓度的增加逐渐增加,浓度为500mg/L时达最高值(97%),此后随着浓度的递增孵化率逐渐降低;各浓度梯度处理组的畸形率无显著差异。溴硝醇浓度为300mg/L时,发眼率(78%)和孵化率(95%)最高,畸形率最低(1.3%);甲醛和双氧水处理组发眼率分别为49%、33%,孵化率分别为93%、91%,畸形率分别为1.5%、0.7%,对照组发眼率、孵化率及畸形率分别为11%、72.3%、0.6%。溴硝醇缓释,药性持久,无刺激性气味,建议在鲑鳟受精卵孵化过程中用300mg/L溴硝醇消毒15min,抑制水霉菌滋生,提高受精卵的成活率。
译 名：
Efficacy of Bronopol against Fungus Saprolegnia Infection in Fertilized Eggs of Rainbow Trout(Oncorhynchus mykiss)
作 者：
SUN Peng;WANG Bing-qian;GU Wei;HU Guo;Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences;
关键词：
bronopol;;rainbow trout;;fertilized egg;;Saprolegnia
摘 要：
The effects of various concentrations of bronopol(2-bromo-2-nitropropane-1,3-propanediol), i.e. 0 mg/L(control), 100mg/L, 300 mg/L, 500 mg/L, 700 mg/L, 900 mg/L and 1100 mg/L, against fungus Saprolegnia infection were studied in fertilized eggs of rainbow trout(Oncorhynchus mykiss)by barrel type incubator at spring water temperature of(4.5±1.3℃). Also, the effects of anti-infection of formaldehyde and hydrogen peroxide at a concentration of 1000 mg/L against fungus Saprolegnia infection were comparatively studied. The results showed that the percentage of eyed embryos was increased in fertilized eggs bathed in the bronopol solution for15 min, the peak eyed embryo rate(78%)and hatching rate(95%)and low deformity rate(1.3%)at a bronopol concentration of 300mg/L as an optimal concentration, above which the percentage of eyed embryos was decreased gradually. There was no significant difference in the abnormality rate among all treatment groups. The 49% of eyed embryos and 33% of hatching rate were found in the fertilized eggs exposed to formaldehyde with abnormality rate of 1.5% and 93% of eyed embryos and 91% of hatching rate were observed in the fertilized eggs exposed to hydrogen peroxide with abnormality rate of 0.7%. There were 11% of eyed embryos, 72.3%of hatch rate and 0.6% of abnormality rate in the control group. Because of the sustained-release characteristics of bronopol, inducing the enduring properties and lacking the characteristic pungent smell. The findings indicated that the bronopol at a concentration of 300mg/L led to efficiently resistance to fungus Saprolegnia infection, and to improve the survival rate of the fertilized eggs of salmonids.
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